Rabbit anti-Human SOS1 Polyclonal Antibody | anti-SOS1 antibody

SOS1 Antibody (Center)

Cat. Num. AAA9215834

• Gene Names: SOS1; GF1; HGF; NS4; GGF1; GINGF
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: SOS1; Polyclonal Antibody; SOS1 Antibody (Center); Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab); Son of sevenless homolog 1; SOS-1; anti-SOS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This SOS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 934-962 amino acids from the Central region of human SOS1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1333

• Applicable Applications for anti-SOS1 antibody: Western Blot (WB), ELISA
• Application Notes: WB ~~ 1:2000
• Function: Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity).
• Tissue Location: Expressed in gingival tissues.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-SOS1 Antibody (Center)at 1:2000 dilution + MOLT-4 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size: 152. 5 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-SOS1 antibody

Son of Sevenless 1 protein is an activator of ras proteins by stimulating the exchange of GDP for GTP on ras. Murine Son of Sevenless proteins have been shown to form a complex with activated EGF receptor, coupling tyrosine kinases to ras proteins. The SOS gene was originally isolated in Drosophila. Two mammalian homologues (mSOS1 and mSOS2) have been isolated from a mouse cDNA library and are expressed in a variety of mouse embryo and adult tissues. Human SOS1 and SOS2 homologs have also been isolated. The two mouse homologs are approximately 70% identical in composition. The mammalian SOS1 protein has a highly specific guanine nucleotide exchange activity towards p21ras. In epidermal growth factor stimulated cells, SOS1 interacts with SH3 domains of GRB2 and binds via SH2 domain to tyrosine 1068 of activated Epidermal Growth Factor receptor.

Product Categories/Family for anti-SOS1 antibody

Cancer; Signal Transduction

References

Roberts A.E., et.al., Nat. Genet. 39:70-74(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 15529996
• NCBI GeneID: 6654
• NCBI Accession #: NP_005624.2
• NCBI GenBank Nucleotide #: NM_005633.3
• UniProt Accession #: Q07889; A8K2G3; B4DXG2
• NCBI Official Full Name: son of sevenless homolog 1
• NCBI Official Synonym Full Names: SOS Ras/Rac guanine nucleotide exchange factor 1
• NCBI Official Symbol: SOS1
• NCBI Official Synonym Symbols: GF1; HGF; NS4; GGF1; GINGF
• NCBI Protein Information: son of sevenless homolog 1
• UniProt Protein Name: Son of sevenless homolog 1
• Protein Family: Son of sevenless
• UniProt Gene Name: SOS1
• UniProt Synonym Gene Names: SOS-1
• UniProt Entry Name: SOS1_HUMAN

NCBI Description

This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008]

Uniprot Description

Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3 in response to EGF (PubMed:17339331). Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity ().