Mouse Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) RTU ELISA Kit | NR3C1 rtu elisa kit

Mouse Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit

Cat. Num. AAA4503587

• Gene Names: Nr3c1; GR; Grl1; Grl-1
• Reactivity: Mouse
• Synonyms: Nuclear Receptor Subfamily 3; Group C; Member 1 (NR3C1); Mouse Nuclear Receptor Subfamily 3; Member 1 (NR3C1) ELISA Kit; GR; GCCR; GCR; GRL; NR3-C1; Glucocorticoid Receptor; NR3C1 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 783

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 33.7pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NR3C1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NR3C1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NR3C1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NR3C1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NR3C1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 121073
• NCBI GeneID: 14815
• UniProt Accession #: P06537; Q61628; Q61629; E0ZPU5
• Molecular Weight: 81,888 Da
• NCBI Official Full Name: Glucocorticoid receptor
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3, group C, member 1
• NCBI Official Symbol: Nr3c1
• NCBI Official Synonym Symbols: GR; Grl1; Grl-1
• NCBI Protein Information: glucocorticoid receptor
• UniProt Protein Name: Glucocorticoid receptor
• Protein Family: Glucocorticoid receptor
• UniProt Gene Name: Nr3c1
• UniProt Synonym Gene Names: Grl; Grl1; GR

Uniprot Description

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:10678832). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth (PubMed:15037546).