Rabbit anti-Human Sorcin/SRI Polyclonal Antibody | anti-SRI antibody

Anti-Sorcin/SRI Antibody, Rabbit Polyclonal

Cat. Num. AAA8107384

• Gene Names: SRI; SCN; V19; CP22; CP-22
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Sorcin/SRI; Polyclonal Antibody; Anti-Sorcin/SRI Antibody; Rabbit Polyclonal; Sorcin/SRI Antibody; Rabbit PAb; Antigen Affinity Purified; sorcin; Anti-CP-22 Antibody; Anti-CP22 Antibody; Anti-SCN Antibody; Anti-V19 Antibody; anti-SRI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Sorcin/SRI
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SRI antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human Sorcin/SRI Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Sorcin/SRI (rh Sorcin/SRI). Sorcin/SRI specific IgG was purified by Human Sorcin/SRI affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SRI was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-SRI rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SRI rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-SRI rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain tissue lysate Lane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:21 kDaObserved band size:18 kDa)

Related Product Information for anti-SRI antibody

Sorcin was originally identified in multidrug-resistant cells. It is a calcium-binding protein. Sorcin modulates excitation-contraction coupling in the heart, contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Sorcin is overexpressed in the multi-drug resistant chinese hamster ovary cell line CHRC5 and a variety of multidrug-resistant tumor cell lines, but overexpression is not a sufficient or necessary condition for the acquisition of the multidrug-resistant phenotype.

References

Meyers MB, et al. (1995) Association of sorcin with the cardiac ryanodine receptor. J Biol Chem. 270(44):26411-8. Brownawell AM, et al. (1997) Calcium-dependent binding of sorcin to the N-terminal domain of synexin (annexin VII). J Biol Chem. 272(35):22182-90. Hansen, et al. (2003) The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Lett. 545(2-3):151-4.

NCBI and Uniprot Product Information

• NCBI GI #: 379317145
• NCBI GeneID: 6717
• NCBI Accession #: NP_001243820.1
• NCBI GenBank Nucleotide #: NM_001256891.1
• UniProt Accession #: P30626; A8MTH6; B4DKK2; D6W5Q0
• Molecular Weight: 20,006 Da
• NCBI Official Full Name: sorcin isoform C
• NCBI Official Synonym Full Names: sorcin
• NCBI Official Symbol: SRI
• NCBI Official Synonym Symbols: SCN; V19; CP22; CP-22
• NCBI Protein Information: sorcin; 22 kDa protein; H_RG167B05.1; calcium binding protein amplified in mutlidrug-resistant cells
• UniProt Protein Name: Sorcin
• UniProt Gene Name: SRI
• UniProt Synonym Gene Names: CP22
• UniProt Entry Name: SORCN_HUMAN

NCBI Description

This gene encodes a calcium-binding protein with multiple E-F hand domains that relocates from the cytoplasm to the sarcoplasmic reticulum in response to elevated calcium levels. In addition to regulating intracellular calcium homeostasis it also modulates excitation-contraction coupling in the heart. Alternative splicing results in multiple transcript variants encoding distinct proteins. Multiple pseudogenes exist for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

SRI: Calcium-binding protein that modulates excitation- contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; smooth endoplasmic reticulum; sarcoplasmic reticulum; mitochondrion; membrane; cytoplasm; T-tubule; nerve terminal; cytosol; Z disc

Molecular Function: protein binding; protease binding; protein heterodimerization activity; calcium channel regulator activity; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; receptor binding

Biological Process: regulation of action potential; cytoplasmic sequestering of transcription factor; muscle development; negative regulation of heart rate; heart development; regulation of striated muscle contraction; regulation of calcium ion transport; signal transduction; proteolysis; regulation of heart contraction; transport; calcium ion transport; positive regulation of release of sequestered calcium ion into cytosol; intracellular sequestering of iron ion