Western Blot (WB)
(Western Blot analysis of Mouse lung tissue using SNAP25 Polyclonal Antibody at dilution of 1:500)
Rabbit anti-Mouse SNAP25 Polyclonal Antibody | anti-SNAP25 antibody
SNAP25 Polyclonal Antibody
Gene Names
Snap25; sp; Bdr; SUP; GENA70; SNAP-25
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
SNAP25; Polyclonal Antibody; SNAP25 Polyclonal Antibody; bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNP 25; SNP25; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein; 25-KD; anti-SNAP25 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Concentration
2 mg/mL (varies by lot)
Sequence Length
206
Applicable Applications for anti-SNAP25 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant Mouse Synaptosomal-associated protein 25 protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of Mouse lung tissue using SNAP25 Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of Mouse lung tissue using SNAP25 Polyclonal Antibody at dilution of 1:500)
Related Product Information for anti-SNAP25 antibody
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.
Product Categories/Family for anti-SNAP25 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 23 kDa
Observed: 23 kDa
Observed: 23 kDa
NCBI Official Full Name
synaptosomal-associated protein 25 isoform b
NCBI Official Synonym Full Names
synaptosomal-associated protein 25
NCBI Official Symbol
Snap25
NCBI Official Synonym Symbols
sp; Bdr; SUP; GENA70; SNAP-25
NCBI Protein Information
synaptosomal-associated protein 25
UniProt Protein Name
Synaptosomal-associated protein 25
Protein Family
UniProt Gene Name
Snap25
UniProt Synonym Gene Names
Snap; SNAP-25; SUP
Uniprot Description
t-SNARE involved in the molecular regulation of neurotransmitter release (PubMed:8243676, PubMed:8103915). May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF (PubMed:16672379). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells ().
Research Articles on SNAP25
Similar Products
Product Notes
The SNAP25 snap25 (Catalog #AAA2554026) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNAP25 Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SNAP25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNAP25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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