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SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

SNAP25 (Synaptosomal-associated protein) Recombinant Protein | SNAP25 recombinant protein

SNAP25 (Synaptosomal-associated protein), 1-206 aa, Human, His tag, E Coli

Gene Names
SNAP25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
SNAP25 (Synaptosomal-associated protein); 1-206 aa; Human; His tag; E Coli; Synaptosomal-associated protein 25 isoform SNAP25B; SNAP25; SNAP; Synaptosomal-associated protein; His- tag; SNAP-25; Synaptosomal-associated 25 kDa protein; Super protein; SuP; Synaptosomal-associated protein 25; Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2; Bdr; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC9; SNAP 25; SNAP-25B; SNP 25; sp; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal Associated Protein 25; Synaptosomal associated protein 25kDa.; SNAP25 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 50mM HEPES pH7.4, 5mM DTT, 100mM NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG
Sequence Length
206
Applicable Applications for SNAP25 recombinant protein
SDS-PAGE
Tag Information
His-tag
Domain
1-206aa
Preparation and Storage
Can be stored at 2°C to 8°C for 1 week.
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

SDS-Page (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for SNAP25 recombinant protein
The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for SNAP25 recombinant protein
References
Tafoya LC., et al. (2006) J. Neurosci. 26 (30):7826-38.; Schulz JR.,. (1998) J. Biol Chem. 273 (38):24355-9.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.4 kDa (226 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
synaptosomal-associated protein 25 isoform SNAP25B
NCBI Official Synonym Full Names
synaptosome associated protein 25
NCBI Official Symbol
SNAP25
NCBI Official Synonym Symbols
SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
NCBI Protein Information
synaptosomal-associated protein 25
UniProt Protein Name
Synaptosomal-associated protein 25
UniProt Gene Name
SNAP25
UniProt Synonym Gene Names
SNAP; SNAP-25; SUP

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

Research Articles on SNAP25

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Product Notes

The SNAP25 snap25 (Catalog #AAA203015) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SNAP25 (Synaptosomal-associated protein) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG. It is sometimes possible for the material contained within the vial of "SNAP25 (Synaptosomal-associated protein), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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