Rabbit anti-Human SMC3 Polyclonal Antibody | anti-SMC3 antibody

SMC3, ID (SMC3, BAM, BMH, CSPG6, SMC3L1, Structural maintenance of chromosomes protein 3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide) (MaxLight 490)

Cat. Num. AAA6348919

• Gene Names: SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SMC3; Polyclonal Antibody; ID (SMC3; BAM; BMH; CSPG6; SMC3L1; Structural maintenance of chromosomes protein 3; Basement membrane-associated chondroitin proteoglycan; Chondroitin sulfate proteoglycan 6; Chromosome-associated polypeptide) (MaxLight 490); Chromosome-associated polypeptide; anti-SMC3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SMC3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-SMC3 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 402-430 in the central region of human SMC3, UniProt Accession #NP_005436.1; Q9UQE7; P97690. Species sequence homology: bovine, mouse, rat
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) usingMBS649784. This demonstrates that MBS649784 detected the SMC3 protein (arrow).)

Related Product Information for anti-SMC3 antibody

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.

Product Categories/Family for anti-SMC3 antibody

Antibodies; Membrane Proteins

References

1. Pie, J., et al. Am. J. Med. Genet. A 152A (4), 924-929 (2010). 2. Terret, M.E., et al. Nature 462(7270):231-234(2009). 3. Revenkova, E., et al. Hum. Mol. Genet. 18(3):418-427(2009). 4. Ridinger, H., et al. Exp. Mol. Pathol. 86(1):23-31(2009). 5. Mohan, K.V., et al. J. Neurovirol. 15(3):229-237(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4885399
• NCBI GeneID: 9126
• NCBI Accession #: NP_005436.1
• NCBI GenBank Nucleotide #: NM_005445.3
• UniProt Accession #: Q9UQE7; P97690; Q9CW03; O97594
• Molecular Weight: 141,542 Da
• NCBI Official Full Name: structural maintenance of chromosomes protein 3
• NCBI Official Synonym Full Names: structural maintenance of chromosomes 3
• NCBI Official Symbol: SMC3
• NCBI Official Synonym Symbols: BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
• NCBI Protein Information: structural maintenance of chromosomes protein 3
• UniProt Protein Name: Structural maintenance of chromosomes protein 3
• UniProt Gene Name: SMC3
• UniProt Synonym Gene Names: BAM; BMH; CSPG6; SMC3L1; SMC protein 3; SMC-3; Bamacan; hCAP
• UniProt Entry Name: SMC3_HUMAN

NCBI Description

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMC3: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112. Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Belongs to the SMC family. SMC3 subfamily.

Protein type: Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: cohesin complex; spindle pole; nuclear matrix; cohesin core heterodimer; chromosome; cytosol; nucleoplasm; lateral element; cytoplasm; basement membrane; chromatin; nucleus; chromosome, pericentric region

Molecular Function: dynein binding; protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding

Biological Process: mitosis; meiosis; mitotic spindle organization and biogenesis; cell division; mitotic sister chromatid cohesion; stem cell maintenance; sister chromatid cohesion; negative regulation of DNA endoreduplication; mitotic cell cycle; signal transduction; DNA repair; regulation of DNA replication

Disease: Cornelia De Lange Syndrome 3