Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: THEMIS2 antibody at 4.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73, 30, 57, 14, 58, 51 KDaObserved band size: 57 KDa)
Rabbit anti-Human THEMIS2 Polyclonal Antibody | anti-THEMIS2 antibody
THEMIS2 Antibody
Gene Names
THEMIS2; ICB-1; C1orf38
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
THEMIS2; Polyclonal Antibody; THEMIS2 Antibody; Protein THEMIS2; Induced by contact to basement membrane 1 protein; Protein ICB-1; Thymocyte-expressed molecule involved in selection protein 2; C1orf38; ICB1; anti-THEMIS2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
123
Applicable Applications for anti-THEMIS2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:200-1:500
IF: 1:50-1:200
IHC: 1:200-1:500
IF: 1:50-1:200
Immunogen
Recombinant Human Protein THEMIS2 protein (62-177AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: THEMIS2 antibody at 4.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73, 30, 57, 14, 58, 51 KDaObserved band size: 57 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysateAll lanes: THEMIS2 antibody at 4.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 73, 30, 57, 14, 58, 51 KDaObserved band size: 57 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7110223 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7110223 diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(Immunofluorescence staining of NIH/3T3 cells with MBS7110223 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC)
(Immunofluorescence staining of NIH/3T3 cells with MBS7110223 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-THEMIS2 antibody
May constitute a control point in macrophage inflammatory response, promoting LPS-induced TNF production.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,028 Da
NCBI Official Full Name
protein THEMIS2 isoform 2
NCBI Official Synonym Full Names
thymocyte selection associated family member 2
NCBI Official Symbol
THEMIS2
NCBI Official Synonym Symbols
ICB-1; C1orf38
NCBI Protein Information
protein THEMIS2
UniProt Protein Name
Protein THEMIS2
Protein Family
UniProt Gene Name
THEMIS2
UniProt Synonym Gene Names
C1orf38; ICB1; Protein ICB-1
Uniprot Description
May constitute a control point in macrophage inflammatory response, promoting LPS-induced TNF production.
Research Articles on THEMIS2
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Product Notes
The THEMIS2 themis2 (Catalog #AAA7110223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The THEMIS2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's THEMIS2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:200-1:500 IF: 1:50-1:200. Researchers should empirically determine the suitability of the THEMIS2 themis2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THEMIS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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