Chromatin Immunoprecipitation (ChIP)
(SMC1-a antibody (pAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from mouse bone marrow cell chromatin and 4 ug SMC1-a antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6.9 million sequence tags were mapped to identify SMC1-a binding sites.)
Rabbit anti-Human SMC1-a Polyclonal Antibody | anti-SMC1-a antibody
SMC1-a antibody (pAb)
ChIP-Seq: 4ug (7ul) per ChIP
IP: 10ul per IP
WB: 1:1,000-1:5,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Chromatin Immunoprecipitation (ChIP)
(SMC1-a antibody (pAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from mouse bone marrow cell chromatin and 4 ug SMC1-a antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6.9 million sequence tags were mapped to identify SMC1-a binding sites.)
Chromatin Immunoprecipitation (ChIP)
(SMC1-a antibody (pAb) tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 15 ug of chromatin from mouse bone marrow cell chromatin and 4 ug SMC1-a antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6.9 million sequence tags were mapped to identify SMC1-a binding sites.)
Western Blot (WB)
(SMC1- a antibody (pAb) tested by Immunoprecipitation. 10 ul of SMC1- a antibody was used to immunoprecipitate SMC1- a from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMC1- a antibody at a dilution of 1:5,000.)
Western Blot (WB)
(SMC1- a antibody (pAb) tested by Immunoprecipitation. 10 ul of SMC1- a antibody was used to immunoprecipitate SMC1- a from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SMC1- a antibody at a dilution of 1:5,000.)
Western Blot (WB)
(SMC1-a antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of HeLa cells probed with SMC1-a antibody at a dilution of 1:5,000.)
Western Blot (WB)
(SMC1-a antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of HeLa cells probed with SMC1-a antibody at a dilution of 1:5,000.)
Background: The Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. SMC1 has a myosin-like ATPase domain that serves as a molecular motor to help organize chromatin and is part of the cohesin complex that facilitates chromosome cohesion during the cell cycle. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. SMC1 is also involved in DNA damage repair. Subsequent to double strand DNA breaks, SMC1 is phosphorylated by the ATM kinase. Phosphorylated SMC1 is crucial to the successful repair of DNA damage. Defects in SMC1 isoform A are the cause of Cornelia de Lange syndrome type 2, an inherited developmental disorder associated with malformations affecting multiple systems.
NCBI and Uniprot Product Information
Uniprot Description
Smc1: a component of the cohesin multiprotein complex that is required for sister chromatid cohesion. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Thought to be an important part of functional kinetochores. Interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.
Protein type: RNA splicing; DNA replication
Chromosomal Location of Human Ortholog: Xp11.22-p11.21
Cellular Component: kinetochore; nucleoplasm; cohesin core heterodimer; condensed nuclear chromosome; cytoplasm; nucleolus; chromosome; nucleus; cytosol; chromosome, pericentric region
Molecular Function: protein binding; protein heterodimerization activity; microtubule motor activity; chromatin binding; ATP binding
Biological Process: meiosis; mitotic sister chromatid segregation; mitotic cell cycle checkpoint; RNA splicing; stem cell maintenance; mitotic sister chromatid cohesion; DNA damage response, signal transduction; sister chromatid cohesion; negative regulation of DNA endoreduplication; DNA repair; nuclear mRNA splicing, via spliceosome; response to radiation; mitotic spindle organization and biogenesis; cell division; gene expression; mitotic cell cycle
Disease: Cornelia De Lange Syndrome 2
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Product Notes
The SMC1-a smc1a (Catalog #AAA388743) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMC1-an antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMC1-a can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ChIP-Seq: 4ug (7ul) per ChIP IP: 10ul per IP WB: 1:1,000-1:5,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the SMC1-a smc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMC1-a, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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