Human Forkhead box protein C2 ELISA Kit | FOXC2 elisa kit

Human Forkhead box protein C2 ELISA Kit

Cat. Num. AAA763925

• Gene Names: FOXC2; LD; MFH1; MFH-1; FKHL14
• Reactivity: Human
• Synonyms: Forkhead box protein C2; Human Forkhead box protein C2 ELISA Kit; FOXC2/Forkhead box protein C2/Transcription factor FKH-14/Forkhead-related protein FKHL14/Mesenchyme fork head protein 1/MFH-1 protein; FOXC2 elisa kit

• Reactivity: Human
• Sequence Length: 501

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for FOXC2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human FOXC2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FOXC2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human FOXC2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human FOXC2can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885237
• NCBI GeneID: 2303
• NCBI Accession #: NP_005242.1
• NCBI GenBank Nucleotide #: NM_005251.2
• Molecular Weight: 53,719 Da
• NCBI Official Full Name: forkhead box protein C2
• NCBI Official Synonym Full Names: forkhead box C2
• NCBI Official Symbol: FOXC2
• NCBI Official Synonym Symbols: LD; MFH1; MFH-1; FKHL14
• NCBI Protein Information: forkhead box protein C2
• UniProt Protein Name: Forkhead box protein C2
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXC2
• UniProt Synonym Gene Names: FKHL14; MFH1; MFH-1 protein
• UniProt Entry Name: FOXC2_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXC2: Transcriptional activator. Might be involved in the formation of special mesenchymal tissues. Defects in FOXC2 are the cause of lymphedema hereditary type 2 (LMPH2); also known as Meige lymphedema. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections, and physical impairment. Defects in FOXC2 are a cause of lymphedema-yellow nails (LYYN). LYYN is characterized by yellow, dystrophic, thick and slowly growing nails, associated with lymphedema and respiratory involvement. Lymphedema occurs more often in the lower limbs. It can appear at birth or later in life. Onset generally follows the onset of ungual abnormalities. Defects in FOXC2 are a cause of lymphedema-distichiasis (LYD). LYD is characterized by primary limb lymphedema usually starting at puberty (but in some cases later or at birth) and associated with distichiasis (double rows of eyelashes, with extra eyelashes growing from the Meibomian gland orifices).

Protein type: Apoptosis; Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: nucleus

Molecular Function: chromatin DNA binding; identical protein binding; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: heart development; insulin receptor signaling pathway; lymphangiogenesis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to hormone stimulus

Disease: Lymphedema-distichiasis Syndrome