Western Blot (WB)
(Western blot analysis of ZBTB7B Antibody (C-term) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab.)
Rabbit anti-Human ZBTB7B Polyclonal Antibody | anti-ZBTB7B antibody
ZBTB7B Antibody (C-term)
Western Blot (WB)
(Western blot analysis of ZBTB7B Antibody (C-term) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ZBTB7B Antibody (C-term) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.)
Widom,R.L., Gene 198 (1-2), 407-420 (1997)
Heegaard,A.M., J. Bone Miner. Res. 12 (12), 2050-2060 (1997)
Widom,R.L., Matrix Biol. 20 (7), 451-462 (2001)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
ZBTB7B: Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; ectoderm development; cell differentiation
Research Articles on ZBTB7B
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Product Notes
The ZBTB7B zbtb7b (Catalog #AAA9203984) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 440-469. The ZBTB7B Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB7B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the ZBTB7B zbtb7b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZBTB7B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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