Western Blot (WB)
(Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat SMARCA3 Polyclonal Antibody | anti-HLTF antibody
Goat anti-SMARCA3 Antibody
Western Blot: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1ug/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9): 4575-87).
Western Blot (WB)
(Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.
Protein type: EC 3.6.1.-; EC 6.3.2.19; Ligase; Ubiquitin ligase; DNA-binding; Nucleolus; Ubiquitin conjugating system; EC 6.3.2.-; EC 3.6.4.-; Helicase
Chromosomal Location of Human Ortholog: 3q25.1-q26.1
Cellular Component: cytoplasm; membrane; nucleolus; nucleoplasm; nucleus
Molecular Function: ATP binding; ATPase activity; DNA binding; helicase activity; ligase activity; protein binding; ubiquitin protein ligase binding; zinc ion binding
Biological Process: chromatin modification; positive regulation of transcription from RNA polymerase II promoter; postreplication repair; protein polyubiquitination; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
Research Articles on HLTF
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Product Notes
The HLTF hltf (Catalog #AAA420394) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SMARCA3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1ug/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observations (J Biol Chem 1995 Mar 3;270(9): 4575-87). Researchers should empirically determine the suitability of the HLTF hltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KQAKINEIRT LIDL. It is sometimes possible for the material contained within the vial of "SMARCA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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