Rabbit HLTF ELISA Kit | HLTF elisa kit

Rabbit HLTF (Helicase Like Transcription Factor) ELISA Kit

Cat. Num. AAA2509893

• Gene Names: HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
• Reactivity: Rabbit
• Synonyms: HLTF; Rabbit HLTF (Helicase Like Transcription Factor) ELISA Kit; HLTF elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit HLTF. No significant cross-reactivity or interference between Rabbit HLTF and analogues was observed.
• Sequence Length: 1009

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HLTF elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit HLTF concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HLTF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HLTF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HLTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HLTF. You can calculate the concentration of HLTF in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21071054
• NCBI GeneID: 6596
• NCBI Accession #: NP_620636.1
• NCBI GenBank Nucleotide #: NM_139048.2
• UniProt Accession #: Q14527; Q14536; Q16051; Q7KYJ6; Q86YA5; Q92652; Q96KM9; D3DNH3
• Molecular Weight: 99,919 Da
• NCBI Official Full Name: helicase-like transcription factor
• NCBI Official Synonym Full Names: helicase-like transcription factor
• NCBI Official Symbol: HLTF
• NCBI Official Synonym Symbols: ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
• NCBI Protein Information: helicase-like transcription factor; DNA-binding protein/plasminogen activator inhibitor 1 regulator; DNA-binding protein/plasminogen activator inhibitor-1 regulator; RING finger protein 80; SNF2-like 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; sucrose nonfermenting protein 2-like 3; sucrose nonfermenting-like 3
• UniProt Protein Name: Helicase-like transcription factor
• Protein Family: Helicase-like transcription factor
• UniProt Gene Name: HLTF
• UniProt Synonym Gene Names: HIP116A; RNF80; SMARCA3; SNF2L3; ZBU1
• UniProt Entry Name: HLTF_HUMAN

NCBI Description

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.

Protein type: Helicase; EC 6.3.2.-; EC 6.3.2.19; EC 3.6.1.-; EC 3.6.4.-; DNA-binding; Ubiquitin ligase; Nucleolus; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 3q25.1-q26.1

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ATPase activity; helicase activity; ATP binding; ligase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; protein polyubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; postreplication repair; chromatin modification