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Western Blot (WB)
(Western Blot analysis of immunized recombinant Smad1 using MBS601298.)
Mouse Smad1 Monoclonal Antibody | anti-Smp_013060 antibody
Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Smad1; Monoclonal Antibody; Smad1 (MADR1; Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma); Anti -Smad1 (MADR1; anti-Smp_013060 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C301
Specificity
Recognizes human Smad1 at ~52kD. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Applicable Applications for anti-Smp_013060 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Flow Cytometry, Dot Blot, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Flow Cytometry: 0.5-2ug/ml. HeLa cells were fixed with 2% paraformaldehyde/PBS and permeabilized in 90% methanol.
Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug/sample
Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS.
Dilution: Flow Cytometry: 0.5-2ug/ml. HeLa cells were fixed with 2% paraformaldehyde/PBS and permeabilized in 90% methanol.
Western Blot: 0.2-2ug/ml
Immunoprecipitation: 100-500ug/sample
Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS.
Immunogen
Partial sequence of recombinant full-length human Smad1 protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of immunized recombinant Smad1 using MBS601298.)
Western Blot (WB)
(Western Blot analysis of immunized recombinant Smad1 using MBS601298.)
Western Blot (WB)
(Detection of SMAD1 by Western Blot using MBS601298. Samples: Whole cell lysate from human HEK293 (H, 25ug), mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 52kD)
Western Blot (WB)
(Detection of SMAD1 by Western Blot using MBS601298. Samples: Whole cell lysate from human HEK293 (H, 25ug), mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 52kD)
Immunoprecipitation (IP)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS601298)
Immunoprecipitation (IP)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with MBS601298)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS601298 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG.)
Testing Data
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with MBS601298 (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG.)
Immunostaining (IS)
(Immunostaining analysis: HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with MBS601298.)
Immunostaining (IS)
(Immunostaining analysis: HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with MBS601298.)
Related Product Information for anti-Smp_013060 antibody
Smad1 belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
Product Categories/Family for anti-Smp_013060 antibody
NCBI and Uniprot Product Information
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Product Notes
The Smp_013060 smad1 (Catalog #AAA601298) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Smad1 (MADR1, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Smad1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Gel Shift Assay (GS/EMSA). Suitable for use in Flow Cytometry, Dot Blot, Western Blot, Immunoprecipitation and Immunocytochemistry. Dilution: Flow Cytometry: 0.5-2ug/ml. HeLa cells were fixed with 2% paraformaldehyde/PBS and permeabilized in 90% methanol. Western Blot: 0.2-2ug/ml Immunoprecipitation: 100-500ug/sample Immunocytochemistry: 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Researchers should empirically determine the suitability of the Smp_013060 smad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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