Goat MTHFR Polyclonal Antibody | anti-MTHFR antibody

Goat Polyclonal to Human MTHFR

Cat. Num. AAA248440

• Reactivity: Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MTHFR; Polyclonal Antibody; Goat Polyclonal to Human MTHFR; Anti-MTHFR Antibody (C-Terminus) IHC-plus; Human MTHFR; anti-MTHFR antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human MTHFR.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 656

• Applicable Applications for anti-MTHFR antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Preliminary experiments gave an approx 28kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2 ug/ml antibody staining. Please note that currently...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-NRPTQNARETEAP from the C-terminus of human MTHFR (NP_005948.3). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey (92%).
• Immunogen Type: Synthetic peptide
• Immunogen: MTHFR antibody was raised against synthetic peptide C-NRPTQNARETEAP from the C-terminus of human MTHFR (NP_005948.3). Percent identity by BLAST analysis: Human, Gorilla (100%); Monkey (92%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MTHFR antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

NCBI and Uniprot Product Information

• NCBI GI #: 87240000
• NCBI GeneID: 4524
• NCBI Accession #: NP_005948.3
• NCBI GenBank Nucleotide #: NM_005957.4
• UniProt Accession #: P42898; Q5SNW6; Q5SNW9; Q7Z6M6; Q8IU73; Q9UQR2; B2R7A6
• Molecular Weight: 78,965 Da
• NCBI Official Full Name: methylenetetrahydrofolate reductase
• NCBI Official Synonym Full Names: methylenetetrahydrofolate reductase (NAD(P)H)
• NCBI Official Symbol: MTHFR
• NCBI Protein Information: methylenetetrahydrofolate reductase; 5,10-methylenetetrahydrofolate reductase (NADPH)
• UniProt Protein Name: Methylenetetrahydrofolate reductase
• Protein Family: Methylenetetrahydrofolate reductase
• UniProt Gene Name: MTHFR
• UniProt Entry Name: MTHR_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq, Oct 2009]

Uniprot Description

MTHFR: Catalyzes the conversion of 5,10- methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co- substrate for homocysteine remethylation to methionine. Defects in MTHFR are the cause of methylenetetrahydrofolate reductase deficiency (MTHFRD). MTHFRD is autosomal recessive disorder with a wide range of features including homocysteinuria, homocysteinemia, developmental delay, severe mental retardation, perinatal death, psychiatric disturbances, and later-onset neurodegenerative disorders. Defects in MTHFR may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in MTHFR may be a cause of susceptibility to folate-sensitive neural tube defects (FS-NTD). The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Belongs to the methylenetetrahydrofolate reductase family.

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; Energy Metabolism - methane; EC 1.5.1.20

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: neuron projection; cytosol

Molecular Function: FAD binding; methylenetetrahydrofolate reductase (NADPH) activity; NADP binding

Biological Process: response to drug; amino acid metabolic process; vitamin metabolic process; blood circulation; methionine biosynthetic process; S-adenosylmethionine metabolic process; one-carbon compound metabolic process; homocysteine metabolic process; response to folic acid; response to vitamin B2; response to hypoxia; folic acid metabolic process; water-soluble vitamin metabolic process

Disease: Schizophrenia; Thrombophilia Due To Thrombin Defect; Neural Tube Defects, Folate-sensitive; Homocystinuria Due To Deficiency Of N(5,10)-methylenetetrahydrofolate Reductase Activity