ACPL2 Recombinant Protein | PXYLP1 recombinant protein

ACPL2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120756

• Gene Names: PXYLP1; XYLP; ACPL2; HEL124
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ACPL2; ACPL2 Protein; Human; Recombinant (His Tag); Human ACPL2 Protein (His Tag); FLJ23751 Protein; UNQ370/PRO706 Protein; PXYLP1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe480

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 24
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human ACPL2 (NP_689495.1)(Met 1-Phe 480) precursor was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PXYLP1 recombinant protein

Background: acid phosphatase-like protein 2, also known as ACPL2, is a secreted protein which belongs to thehistidine acid phosphatase family. A large-scale effort, termed the Secreted Protein Discovery Initiative (SPDI), was undertaken to identify novel secreted and transmembrane proteins. In the first of several approaches, a biological signal sequence trap in yeast cells was utilized to identify cDNA clones encoding putative secreted proteins. A second strategy utilized various algorithms that recognize features such as the hydrophobic properties of signal sequences to identify putative proteins encoded by expressed sequence tags (ESTs) from human cDNA libraries. A third approach surveyed ESTs for protein sequence similarity to a set of known receptors and their ligands with the BLAST algorithm. Finally, both signal-sequence prediction algorithms and BLAST were used to identify single exons of potential genes from within human genomic sequence.

References

Clark HF., et al., 2003, Genome Res. 13: 2265-70. Ota T.et al., 2004, Nat. Genet. 36:40-5.

NCBI and Uniprot Product Information

• NCBI GI #: 22748649
• NCBI GeneID: 92370
• NCBI Accession #: NP_689495.1
• NCBI GenBank Nucleotide #: NM_152282.4
• UniProt Accession #: Q8TE99; Q49AJ2; D3DNF5; W0TR04
• Molecular Weight: 53,257 Da
• NCBI Official Full Name: 2-phosphoxylose phosphatase 1 isoform 1
• NCBI Official Synonym Full Names: 2-phosphoxylose phosphatase 1
• NCBI Official Symbol: PXYLP1
• NCBI Official Synonym Symbols: XYLP; ACPL2; HEL124
• NCBI Protein Information: 2-phosphoxylose phosphatase 1
• UniProt Protein Name: 2-phosphoxylose phosphatase 1
• Protein Family: 2-phosphoxylose phosphatase
• UniProt Gene Name: PXYLP1

Uniprot Description

Responsible for the 2-O-dephosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature glycosaminoglycan (GAG) chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG-protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1-O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-O-dephosphorylation during completion of linkage region formation is a prerequisite for the initiation and efficient elongation of the repeating disaccharide region of GAG chains.