Rabbit anti-Mouse SLIT1 Polyclonal Antibody | anti-SLIT1 antibody

SLIT1, NT (SLIT1, KIAA0813, MEGF4, SLIL1, Slit homolog 1 protein, Multiple epidermal growth factor-like domains protein 4) (AP)

Cat. Num. AAA6348649

• Gene Names: SLIT1; MEGF4; SLIL1; SLIT3; SLIT-1
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLIT1; Polyclonal Antibody; NT (SLIT1; KIAA0813; MEGF4; SLIL1; Slit homolog 1 protein; Multiple epidermal growth factor-like domains protein 4) (AP); Multiple epidermal growth factor-like domains protein 4; anti-SLIT1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SLIT1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SLIT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-113 amino acids from the N-terminal region of human SLIT1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SLIT1 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SLIT1 antibody detected the SLIT1 protein (arrow).)

Immunohistochemistry (IHC)

(SLIT1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLIT1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SLIT1 antibody

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.

Product Categories/Family for anti-SLIT1 antibody

Antibodies; Growth Factors; Cytokines

References

Marlow, R., et al. Cancer Res. 68(19):7819-7827(2008). Hussain, S.A., et al. J. Biol. Chem. 281(51):39693-39698(2006). Lin, L., et al. Stem Cells 24(11):2504-2513(2006). Lim, J., et al. Cell 125(4):801-814(2006). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 188528675
• NCBI GeneID: 6585
• NCBI Accession #: NP_003052.2
• NCBI GenBank Nucleotide #: NM_003061.2
• UniProt Accession #: O75093; Q5T0V1; Q8WWZ2; Q9UIL7
• Molecular Weight: 90,633 Da
• NCBI Official Full Name: slit homolog 1 protein
• NCBI Official Synonym Full Names: slit homolog 1 (Drosophila)
• NCBI Official Symbol: SLIT1
• NCBI Official Synonym Symbols: MEGF4; SLIL1; SLIT3; SLIT-1
• NCBI Protein Information: slit homolog 1 protein; multiple EGF-like domains protein 4; multiple epidermal growth factor-like domains protein 4
• UniProt Protein Name: Slit homolog 1 protein
• Protein Family: Slit homolog 1 protein
• UniProt Gene Name: SLIT1
• UniProt Synonym Gene Names: KIAA0813; MEGF4; SLIL1; Slit-1; Multiple EGF-like domains protein 4

Uniprot Description

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.