Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (15ug/lane) using MBS648429. This demonstrates that MBS648429 detected the SLC4A7 protein (arrow).)
Rabbit anti-Human SLC4A7 Polyclonal Antibody | anti-SLC4A7 antibody
SLC4A7, CT (SLC4A7, BT, NBC2, NBC2B, NBC3, SBC2, SLC4A6, Sodium bicarbonate cotransporter 3, Sodium bicarbonate cotransporter 2, Sodium bicarbonate cotransporter 2b, Solute carrier family 4 member 7)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (15ug/lane) using MBS648429. This demonstrates that MBS648429 detected the SLC4A7 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in K562 cell line lysates (15ug/lane) using MBS648429. This demonstrates that MBS648429 detected the SLC4A7 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue using MBS648429 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648429 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue using MBS648429 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648429 for immunohistochemistry.)
NCBI and Uniprot Product Information
NCBI Description
This locus encodes a sodium bicarbonate cotransporter. The encoded transmembrane protein appears to transport sodium and bicarbonate ions in a 1:1 ratio, and is thus considered an electroneutral cotransporter. The encoded protein likely plays a critical role in regulation of intracellular pH involved in visual and auditory sensory transmission. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]
Uniprot Description
Function: Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na+:HCO3- 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity. Ref.2 Ref.9
Enzyme regulation: Transporter activity is regulated by CA2/carbonic anhydrase 2, cAMP and PKA. Insensitive to stilbene derivatives. Ref.2 states it is inhibited by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA).
Subunit structure: Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C. Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2. Ref.9 Ref.10 Ref.11 Ref.13
Subcellular location: Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein
By similarity. Cell projection › stereocilium
By similarity. Note: Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells
By similarity.
Tissue specificity: Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle. Ref.1
Domain: The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C. Ref.9
Sequence similarities: Belongs to the anion exchanger (TC 2.A.31) family. [View classification]
Biophysicochemical propertiesKinetic parameters:KM=24 mM for external sodium Ref.2
Research Articles on SLC4A7
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Product Notes
The SLC4A7 slc4a7 (Catalog #AAA648429) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC4A7, CT (SLC4A7, BT, NBC2, NBC2B, NBC3, SBC2, SLC4A6, Sodium bicarbonate cotransporter 3, Sodium bicarbonate cotransporter 2, Sodium bicarbonate cotransporter 2b, Solute carrier family 4 member 7) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SLC4A7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the SLC4A7 slc4a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC4A7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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