Rabbit anti-Human SLC4A7 Polyclonal Antibody | anti-SLC4A7 antibody

SLC4A7, CT (SLC4A7, BT, NBC2, NBC2B, NBC3, SBC2, SLC4A6, Sodium bicarbonate cotransporter 3, Sodium bicarbonate cotransporter 2, Sodium bicarbonate cotransporter 2b, Solute carrier family 4 member 7) (MaxLight 650)

Cat. Num. AAA6348492

• Gene Names: SLC4A7; NBC2; NBC3; SBC2; NBCN1; SLC4A6
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC4A7; Polyclonal Antibody; CT (SLC4A7; BT; NBC2; NBC2B; NBC3; SBC2; SLC4A6; Sodium bicarbonate cotransporter 3; Sodium bicarbonate cotransporter 2; Sodium bicarbonate cotransporter 2b; Solute carrier family 4 member 7) (MaxLight 650); Solute carrier family 4 member 7; anti-SLC4A7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLC4A7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-SLC4A7 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1186-1214 in the C-terminal region of human SLC4A7, UniProt Accession #Q9Y6M7; NP_003606.3.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (15ug/lane) usingMBS648429. This demonstrates that MBS648429 detected the SLC4A7 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue using MBS648429 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS648429 for immunohistochemistry.)

Related Product Information for anti-SLC4A7 antibody

SLC4A7 regulates intracellular pH and may also play a role in bicarbonate salvage in secretory epithelia. Additionally, SLC4A7 may also have associated sodium channel activity.

Product Categories/Family for anti-SLC4A7 antibody

Antibodies; Transport Proteins

References

1. Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010). 2. Ahmed, S., et al. Nat. Genet. 41(5):585-590(2009). 3. Ishiguro, H., et al. Addiction 102(8):1320-1325(2007). 4. Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007). 5. Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 134288865
• NCBI GeneID: 9497
• NCBI Accession #: NP_003606.3
• NCBI GenBank Nucleotide #: NM_003615.4
• UniProt Accession #: Q9Y6M7; A6NIA8; B2CI53; B5M449; B5M451; B5M452; B5M453; B6DY52; B6DY53; C9JST9; D3K174; D3K175
• Molecular Weight: 52,692 Da
• NCBI Official Full Name: sodium bicarbonate cotransporter 3 isoform a
• NCBI Official Synonym Full Names: solute carrier family 4 member 7
• NCBI Official Symbol: SLC4A7
• NCBI Official Synonym Symbols: NBC2; NBC3; SBC2; NBCN1; SLC4A6
• NCBI Protein Information: sodium bicarbonate cotransporter 3
• UniProt Protein Name: Sodium bicarbonate cotransporter 3
• Protein Family: Sodium bicarbonate cotransporter
• UniProt Gene Name: SLC4A7
• UniProt Synonym Gene Names: BT; NBC2; NBC2B; NBC3; NBCn1; SBC2; SLC4A6; Bicarbonate transporter
• UniProt Entry Name: S4A7_HUMAN

NCBI Description

This locus encodes a sodium bicarbonate cotransporter. The encoded transmembrane protein appears to transport sodium and bicarbonate ions in a 1:1 ratio, and is thus considered an electroneutral cotransporter. The encoded protein likely plays a critical role in regulation of intracellular pH involved in visual and auditory sensory transmission. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]

Uniprot Description

SLC4A7: Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity. Belongs to the anion exchanger (TC 2.A.31) family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p22

Cellular Component: stereocilium; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: inorganic anion exchanger activity; sodium:bicarbonate symporter activity

Biological Process: cochlear nucleus development; bicarbonate transport; regulation of intracellular pH; auditory receptor cell development; retinal cell programmed cell death; ion transport; transmembrane transport