Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SAMSN1 Polyclonal Antibody | anti-SAMSN1 antibody
Goat anti-SAMSN1 Antibody
Gene Names
SAMSN1; SLy2; HACS1; NASH1; SASH2; SH3D6B
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SAMSN1; Polyclonal Antibody; Goat anti-SAMSN1 Antibody; HACS1; SAM domain; SH3 domain and nuclear localisation signals; 1; SH3-SAM adaptor protein; hematopoietic adaptor containing SH3 and SAM domains 1; NASH1; SASH2; SH3D6B; SH3 domain and nuclear localization signals 1; SAM and SH3 domain; SAMSN1 antibody; HACS1 antibody; 1 antibody; SH3-SAM adaptor protein antibody; hematopoietic adaptor containing SH3 and SAM domains 1 antibody; NASH1 antibody; SASH2 antibody; SH3 domain and nuclear localization signals 1 antibody; SAM and SH3 domain containing 2 antibody; SH3 domain and nuclear localization signals; nuclear localization signals; SAM and SH3 domain containing 1 antibody; anti-SAMSN1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
IRLDTLSASLGRSC
Sequence Length
441
Applicable Applications for anti-SAMSN1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 48kDa band observed in Jurkat Tcell lysate (calculated MW of 41.7kDa according to NP_071419.2). The observed size is consistent with observations with competing products. Recommended concentration: 0.5-1.5ug/ml.
Western Blot: Approx 48kDa band observed in Jurkat Tcell lysate (calculated MW of 41.7kDa according to NP_071419.2). The observed size is consistent with observations with competing products. Recommended concentration: 0.5-1.5ug/ml.
Immunogen
Peptide with sequence IRLDTLSASLGRSC, from the N Terminus of the protein sequence according to NP_071419.2.
Epitope
N Terminus
Note
Please note that the immunogen sequence is present in NP_071419.2 (and in AAK07746) but is no longer present in the latest reference sequence (NP_071419.3 at 05/10/05). This latest version has not been subject to final review by NCBI. This antibody may be recognising one particular isoform.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,627 Da
NCBI Official Full Name
SAM domain, SH3 domain and nuclear localisation signals, 1; SAMSN1 protein
NCBI Official Synonym Full Names
SAM domain, SH3 domain and nuclear localization signals 1
NCBI Official Symbol
SAMSN1
NCBI Official Synonym Symbols
SLy2; HACS1; NASH1; SASH2; SH3D6B
NCBI Protein Information
SAM domain-containing protein SAMSN-1
UniProt Protein Name
SAM domain-containing protein SAMSN-1
Protein Family
UniProt Gene Name
SAMSN1
UniProt Synonym Gene Names
HACS1
UniProt Entry Name
SAMN1_HUMAN
NCBI Description
SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains (Claudio et al., 2001 [PubMed 11536050]).[supplied by OMIM, Mar 2008]
Uniprot Description
SAMSN1: an adaptor and scaffold protein containing an SH3 and a SAM (sterile alpha motif) domain. Its expression is induced by B cell activation signals. Two alternatively spliced isoforms have been described.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 21q11
Cellular Component: cytosol; nucleus; ruffle
Molecular Function: phosphotyrosine binding
Biological Process: negative regulation of adaptive immune response; negative regulation of B cell activation; negative regulation of peptidyl-tyrosine phosphorylation
Research Articles on SAMSN1
Similar Products
Product Notes
The SAMSN1 samsn1 (Catalog #AAA420016) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SAMSN1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAMSN1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 48kDa band observed in Jurkat Tcell lysate (calculated MW of 41.7kDa according to NP_071419.2). The observed size is consistent with observations with competing products. Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the SAMSN1 samsn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IRLDTLSASL GRSC. It is sometimes possible for the material contained within the vial of "SAMSN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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