Rabbit anti-Human SLC38A9 Polyclonal Antibody | anti-SLC38A9 antibody

SLC38A9 Antibody

Cat. Num. AAA7044112

• Gene Names: SLC38A9; URLC11
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: SLC38A9; Polyclonal Antibody; SLC38A9 Antibody; Sodium-coupled neutral amino acid transporter 9 Curated; Solute carrier family 38 member 9; anti-SLC38A9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 498

• Applicable Applications for anti-SLC38A9 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:5000; IHC: 1:20 - 1:200; IF: 1:50 - 1:200
• Immunogen Species: Homo Sapiens (Human)
• Immunogen: Recombinant human Sodium-coupled neutral amino acid transporter 9 protein (1-119AA)
• Target Names: SLC38A9
• Research Area: Signal Transduction
• Conjugate: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Jurkat whole cell lysateAll lanes: SLC38A9 antibody at 3.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 64,53,57 kDaObserved band size: 64 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver tissue using MBS7044112 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7044112 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of U251 cells using MBS7044112 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-SLC38A9 antibody

Lysosomal amino acid transporter involved in the activation of mTORC1 in response to amino acids. Probably acts as an amino acid sensor of the Rag GTPases and Ragulator complexes, 2 complexes involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids (PubMed:25561175, PubMed:25567906). Following activation by amino acids, the Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. SLC38A9 mediates transport of amino acids with low capacity and specificity with a slight preference for polar amino acids, suggesting that it acts as an amino acid sensor instead (PubMed:25561175, PubMed:25567906). The high concentration of arginine in lysosomes suggests that it acts as an arginine sensor (PubMed:25567906).

NCBI and Uniprot Product Information

• NCBI GI #: 385137120
• NCBI GeneID: 153129
• NCBI Accession #: NP_001245215.1
• NCBI GenBank Nucleotide #: NM_001258286.1
• UniProt Accession #: Q8NBW4; Q0P5S0; Q6MZJ8; A0A0A8K8P2; B3KXV1; B7Z7D0
• NCBI Official Full Name: sodium-coupled neutral amino acid transporter 9 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 38 member 9
• NCBI Official Symbol: SLC38A9
• NCBI Official Synonym Symbols: URLC11
• NCBI Protein Information: sodium-coupled neutral amino acid transporter 9
• UniProt Protein Name: Sodium-coupled neutral amino acid transporter 9
• Protein Family: Sodium-coupled neutral amino acid transporter
• UniProt Gene Name: SLC38A9
• UniProt Entry Name: S38A9_HUMAN

Uniprot Description

SLC38A9: Putative sodium-dependent amino acid/proton antiporter. Belongs to the amino acid/polyamine transporter 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter, SLC family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: endoplasmic reticulum; late endosome; lysosome

Molecular Function: amino acid transmembrane transporter activity; protein binding

Biological Process: positive regulation of TOR signaling pathway