Typical Testing Data/Standard Curve (for reference only)
Monkey Zinc finger protein Pegasus (IKZF5) ELISA Kit | IKZF5 elisa kit
Monkey Zinc finger protein Pegasus (IKZF5) ELISA Kit
Gene Names
IKZF5; PEGASUS; ZNFN1A5
Reactivity
Monkey
Synonyms
Zinc finger protein Pegasus (IKZF5); Monkey Zinc finger protein Pegasus (IKZF5) ELISA Kit; IKZF5 elisa kit
Reactivity
Monkey
Specificity
This assay has high sensitivity and excellent specificity for detection of IKZF5. No significant cross-reactivity or interference between IKZF5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IKZF5 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IKZF5 elisa kit
Intended Uses: This IKZF5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IKZF5. This ELISA kit for research use only!
Principle of the Assay: IKZF5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IKZF5 antibody and an IKZF5-HRP conjugate. The assay sample and buffer are incubated together with IKZF5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IKZF5 concentration since IKZF5 from samples and IKZF5-HRP conjugate compete for the anti-IKZF5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IKZF5 from the sample, fewer sites are left to bind IKZF5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKZF5 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IKZF5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IKZF5 antibody and an IKZF5-HRP conjugate. The assay sample and buffer are incubated together with IKZF5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IKZF5 concentration since IKZF5 from samples and IKZF5-HRP conjugate compete for the anti-IKZF5 antibody binding site. Since the number of sites is limited, as more sites are occupied by IKZF5 from the sample, fewer sites are left to bind IKZF5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKZF5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IKZF5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,510 Da
NCBI Official Full Name
zinc finger protein Pegasus
NCBI Official Synonym Full Names
IKAROS family zinc finger 5 (Pegasus)
NCBI Official Symbol
IKZF5
NCBI Official Synonym Symbols
PEGASUS; ZNFN1A5
NCBI Protein Information
zinc finger protein Pegasus; zinc finger transcription factor Pegasus; zinc finger protein, subfamily 1A, 5 (Pegasus)
UniProt Protein Name
Zinc finger protein Pegasus
Protein Family
UniProt Gene Name
IKZF5
UniProt Synonym Gene Names
ZNFN1A5
UniProt Entry Name
IKZF5_HUMAN
NCBI Description
Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Pegasus, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM, Jul 2002]
Uniprot Description
IKZF5: DNA-binding protein that binds to the 5'GNNTGTNG-3' core sequence. Transcriptional repressor. Belongs to the Ikaros C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 10q26
Cellular Component: nucleus
Molecular Function: DNA binding; metal ion binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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Product Notes
The Monkey IKZF5 ikzf5 (Catalog #AAA7224269) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7224269 ELISA Kit recognizes Monkey IKZF5. It is sometimes possible for the material contained within the vial of "Zinc finger protein Pegasus (IKZF5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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