Rabbit anti-Human, Mouse ROBLD3 Polyclonal Antibody | anti-ROBLD3 antibody

ROBLD3 Antibody

Cat. Num. AAA9609338

• Gene Names: LAMTOR2; p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ROBLD3; Polyclonal Antibody; ROBLD3 Antibody; ENDAP; Endosomal adaptor protein p14; HSPC003; LAMTOR2; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Late endosomal/lysosomal Mp1 interacting protein; Late endosomal/lysosomal Mp1-interacting protein; LTOR2_HUMAN; MAPBPIP; MAPKSP1 adaptor protein; MAPKSP1AP; Mitogen activated protein binding protein interacting protein; Mitogen-activated protein-binding protein-interacting protein; p14; Ragulator complex protein LAMTOR2; Ragulator2; Roadblock domain containing 3; Roadblock domain containing protein 3; Roadblock domain-containing protein 3; ROBLD 3; RP11 336K24.9; anti-ROBLD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ROBLD3 antibody detects endogenous levels of total ROBLD3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 95

• Applicable Applications for anti-ROBLD3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200
• Immunogen: A synthesized peptide derived from human ROBLD3
• Subcellular Location: Late Endosome Membrane. Lysosome Membrane.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9609338 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of ROBLD3 using MCF7 whole cell lysates)

Related Product Information for anti-ROBLD3 antibody

Function: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
Subunit Structure: Part of the Ragulator complex composed of LAMTOR1, LAMTOR2, LAMTOR3, LAMTOR4 and LAMTOR5. LAMTOR4 and LAMTOR5 form a heterodimer that interacts, through LAMTOR1, with a LAMTOR2, LAMTOR3 heterodimer (PubMed:20381137, PubMed:22980980). The Ragulator complex interacts with both the mTORC1 complex and heterodimers constituted of the Rag GTPases RRAGA, RRAGB, RRAGC and RRAGD; regulated by amino acid availability (PubMed:20381137, PubMed:22980980). The Ragulator complex interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906). Interacts with LAMTOR1 and LAMTOR3; the interaction is direct. Interacts with MAPK1 and MAP2K1 (By similarity).
Similarity: Belongs to the GAMAD family.

NCBI and Uniprot Product Information

• NCBI GI #: 223671906
• NCBI GeneID: 28956
• NCBI Accession #: NP_001138736.1
• NCBI GenBank Nucleotide #: NM_001145264.1
• UniProt Accession #: Q9Y2Q5; Q5VY97; Q5VY98; Q5VY99
• Molecular Weight: Observed: 14 kDa; Predicted: 14 kDa
• NCBI Official Full Name: ragulator complex protein LAMTOR2 isoform 2
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
• NCBI Official Symbol: LAMTOR2
• NCBI Official Synonym Symbols: p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• NCBI Protein Information: ragulator complex protein LAMTOR2
• UniProt Protein Name: Ragulator complex protein LAMTOR2
• UniProt Gene Name: LAMTOR2
• UniProt Synonym Gene Names: MAPBPIP; ROBLD3; MAPBP-interacting protein

NCBI Description

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.