Rabbit anti-Human, Rat FOXI1 Polyclonal Antibody | anti-FOXI1 antibody

Anti-FOXI1 Antibody

Cat. Num. AAA1753154

• Gene Names: FOXI1; HFH3; FKH10; HFH-3; FKHL10; FREAC6; FREAC-6
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: FOXI1; Polyclonal Antibody; Anti-FOXI1 Antibody; Forkhead box protein I1; Forkhead-related protein FKHL10; Forkhead-related transcription factor 6; FREAC-6; Hepatocyte nuclear factor 3 forkhead homolog 3; HFH-3; HNF-3/fork-head homolog 3; FKHL10; FREAC6; anti-FOXI1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 378

• Applicable Applications for anti-FOXI1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Human); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human FOXI1 recombinant protein (Position: M1-V378).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Western Blot (WB)

(Figure 1. Western blot analysis of FOXI1 using anti-FOXI1 antibody.)

Related Product Information for anti-FOXI1 antibody

Description: Rabbit IgG polyclonal antibody for FOXI1 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Rat.

Background: Forkhead box I1 is a protein that in humans is encoded by the FOXI1 gene. It is mapped to 5q35.1. This gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene may play an important role in the development of the cochlea and vestibulum, as well as in embryogenesis. The encoded protein has been found to be required for the transcription of four subunits of a proton pump found in the inner ear, the kidney, and the epididymis. Mutations in this gene have been associated with deafness, autosomal recessive 4.

References

1. Blomqvist, S. R., Vidarsson, H., Fitzgerald, S., Johansson, B. R., Ollerstam, A., Brown, R., Persson, A. E. G., Bergstrom, G., Enerback, S. Distal renal tubular acidosis in mice that lack the forkhead transcription factor Foxi1. J. Clin. Invest. 113: 1560-1570, 2004.
2. Blomqvist, S. R., Vidarsson, H., Soder, O., Enerback, S. Epididymal expression of the forkhead transcription factor Foxi1 is required for male fertility. EMBO J. 25: 4131-4141, 2006.
3. Hulander, M., Kiernan, A. E., Blomqvist, S. R., Carlsson, P., Samuelsson, E. J., Johansson, B. R., Steel, K. P., Enerback, S. Lack of pendrin expression leads to deafness and expansion of the endolymphatic compartment in inner ears of Foxi1 null mutant mice. Development 130: 2013-2025, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 21618327
• NCBI GeneID: 2299
• NCBI Accession #: NP_036320.2
• NCBI GenBank Nucleotide #: NP_036320.2
• UniProt Accession #: Q12951
• NCBI Official Full Name: forkhead box protein I1 isoform a
• NCBI Official Synonym Full Names: forkhead box I1
• NCBI Official Symbol: FOXI1
• NCBI Official Synonym Symbols: HFH3; FKH10; HFH-3; FKHL10; FREAC6; FREAC-6
• NCBI Protein Information: forkhead box protein I1
• UniProt Protein Name: Forkhead box protein I1
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXI1
• UniProt Synonym Gene Names: FKHL10; FREAC6; FREAC-6; HFH-3; HNF-3/fork-head homolog 3
• UniProt Entry Name: FOXI1_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors, which is characterized by a distinct forkhead domain. This gene may play an important role in the development of the cochlea and vestibulum, as well as in embryogenesis. The encoded protein has been found to be required for the transcription of four subunits of a proton pump found in the inner ear, the kidney, and the epididymis. Mutations in this gene have been associated with deafness, autosomal recessive 4. [provided by RefSeq, Jan 2017]