Rabbit anti-Human SKAP1 Polyclonal Antibody | anti-SKAP1 antibody

SKAP1, CT (SKAP1, SCAP1, SKAP55, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1, Src kinase-associated phosphoprotein of 55kD) (APC)

Cat. Num. AAA6347913

• Gene Names: SKAP1; SCAP1; SKAP55; HEL-S-81p
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SKAP1; Polyclonal Antibody; CT (SKAP1; SCAP1; SKAP55; Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55kD) (APC); Src kinase-associated phosphoprotein of 55kD; anti-SKAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SKAP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 265-295 amino acids selected from the C-terminal region of human SKAP1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SKAP1 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SKAP1 antibody detected the SKAP1 protein (arrow).)

Related Product Information for anti-SKAP1 antibody

This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.

Product Categories/Family for anti-SKAP1 antibody

Antibodies; Protein Kinases

References

Goode, E.L., et al. Nat. Genet. 42(10):874-879(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Raab, M., et al. Immunity 32(4):541-556(2010). Lehmann, R., et al. Proteomics 9(23):5288-5295(2009). Fang, L., et al. Cell Cycle 8(17):2819-2827(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 115527074
• NCBI GeneID: 8631
• NCBI Accession #: NP_003717.3
• NCBI GenBank Nucleotide #: NM_003726.3
• UniProt Accession #: Q86WV1; O15268; D3DTV1
• Molecular Weight: 41,333 Da
• NCBI Official Full Name: src kinase-associated phosphoprotein 1 isoform 1
• NCBI Official Synonym Full Names: src kinase associated phosphoprotein 1
• NCBI Official Symbol: SKAP1
• NCBI Official Synonym Symbols: SCAP1; SKAP55; HEL-S-81p
• NCBI Protein Information: src kinase-associated phosphoprotein 1; pp55; SKAP-55; src family associated phosphoprotein 1; src family-associated phosphoprotein 1; src kinase-associated phosphoprotein of 55 kDa; epididymis secretory sperm binding protein Li 81p
• UniProt Protein Name: Src kinase-associated phosphoprotein 1
• Protein Family: Src kinase-associated phosphoprotein
• UniProt Gene Name: SKAP1
• UniProt Synonym Gene Names: SCAP1; SKAP55; SKAP-55; pp55
• UniProt Entry Name: SKAP1_HUMAN

NCBI Description

This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008]

Uniprot Description

SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; plasma membrane; intercellular junction; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity; protein complex binding; SH2 domain binding; SH3 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway