Chicken anti-Human, Mouse SIVA Polyclonal Antibody | anti-SIVA1 antibody

SIVA Antibody

Cat. Num. AAA150365

• Gene Names: SIVA1; SIVA; CD27BP; Siva-1; Siva-2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: SIVA Antibody is affinity chromatography purified via peptide column.
• Synonyms: SIVA; Polyclonal Antibody; SIVA Antibody; CD27BP; Siva-1; Siva-2; Apoptosis regulatory protein Siva; CD27-binding protein; SIVA1; apoptosis-inducing factor; anti-SIVA1 antibody

• Host: Chicken
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgY
• Purity/Purification: SIVA Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 175

• Applicable Applications for anti-SIVA1 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: SIVA antibody can be used for detection of EPAC1 by Western blot at 1 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: SIVA antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human SIVA.
• Buffer: SIVA Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SIVA antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SIVA in mouse liver tissue lysate with SIVA antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SIVA in mouse liver cells with SIVA antibody at 20 μg/mL.)

Related Product Information for anti-SIVA1 antibody

SIVA Antibody: SIVA plays an important role in the apoptotic pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. SIVA is a small cysteine-rich protein with a unique N-terminal amphipathic helical region, a death domain homology region (DDHR) and a C-terminal cysteine-rich B-box-like or zinc-finger-like structure. The DDHR domain binds to CD27 and induces apoptosis in T cells through a caspase-dependent mitochondrial pathway. SIVA inhibits BCL2L1 isoform Bcl-xL anti-apoptotic activity and inhibits activation of NFκ-B and promotes T-cell receptor-mediated apoptosis. SIVA represents a potential therapeutic target for the treatment of neuronal cell death diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 11277468
• NCBI GeneID: 10572
• NCBI Accession #: NP_068355
• NCBI GenBank Nucleotide #: NM_006427.3
• UniProt Accession #: O15304; Q96P98; Q9UPD6
• Molecular Weight: 11,778 Da
• NCBI Official Full Name: apoptosis regulatory protein Siva isoform 1
• NCBI Official Synonym Full Names: SIVA1, apoptosis-inducing factor
• NCBI Official Symbol: SIVA1
• NCBI Official Synonym Symbols: SIVA; CD27BP; Siva-1; Siva-2
• NCBI Protein Information: apoptosis regulatory protein Siva; CD27-binding (Siva) protein
• UniProt Protein Name: Apoptosis regulatory protein Siva
• Protein Family: Apoptosis regulatory protein
• UniProt Gene Name: SIVA1
• UniProt Synonym Gene Names: SIVA; CD27BP
• UniProt Entry Name: SIVA_HUMAN

NCBI Description

This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Siva: a proapoptotic protein. Associates with CD27, a member of the tumor necrosis factor receptor family. Two alternatively spliced isoforms have been described. Isoform 1 but not isoform 2 may induce TNFRSF7/CD27 mediated apoptosis. Isoform 1 inhibits Bcl-xL anti-apoptotic activity.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: viral receptor activity; protein binding; zinc ion binding; metal ion binding; CD27 receptor binding

Biological Process: entry of virus into host cell; inhibition of NF-kappaB transcription factor; activated T cell apoptosis