Human Apoptosis regulatory protein Siva ELISA Kit | SIVA1 elisa kit

Human Apoptosis regulatory protein Siva ELISA Kit

Cat. Num. AAA761258

• Gene Names: SIVA1; SIVA; CD27BP; Siva-1; Siva-2
• Reactivity: Human
• Synonyms: Apoptosis regulatory protein Siva; Human Apoptosis regulatory protein Siva ELISA Kit; SIVA1/Apoptosis regulatory protein Siva/CD27-binding protein (CD27BP; SIVA1 elisa kit

• Reactivity: Human
• Sequence Length: 110

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SIVA1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human SIVA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SIVA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SIVA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman SIVA1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11277470
• NCBI GeneID: 10572
• NCBI Accession #: NP_068355.1
• NCBI GenBank Nucleotide #: NM_021709.2
• Molecular Weight: 11,778 Da
• NCBI Official Full Name: apoptosis regulatory protein Siva isoform 2
• NCBI Official Synonym Full Names: SIVA1 apoptosis inducing factor
• NCBI Official Symbol: SIVA1
• NCBI Official Synonym Symbols: SIVA; CD27BP; Siva-1; Siva-2
• NCBI Protein Information: apoptosis regulatory protein Siva
• UniProt Protein Name: Apoptosis regulatory protein Siva
• Protein Family: Apoptosis regulatory protein
• UniProt Gene Name: SIVA1
• UniProt Synonym Gene Names: SIVA; CD27BP
• UniProt Entry Name: SIVA_HUMAN

NCBI Description

This gene encodes a protein with an important role in the apoptotic (programmed cell death) pathway induced by the CD27 antigen, a member of the tumor necrosis factor receptor (TFNR) superfamily. The CD27 antigen cytoplasmic tail binds to the N-terminus of this protein. Two alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Siva: a proapoptotic protein. Associates with CD27, a member of the tumor necrosis factor receptor family. Two alternatively spliced isoforms have been described. Isoform 1 but not isoform 2 may induce TNFRSF7/CD27 mediated apoptosis. Isoform 1 inhibits Bcl-xL anti-apoptotic activity.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: cytoplasm; mitochondrion; nucleoplasm

Molecular Function: CD27 receptor binding; metal ion binding; protein binding; viral receptor activity; zinc ion binding

Biological Process: activated T cell apoptosis; entry of virus into host cell; inhibition of NF-kappaB transcription factor