Rabbit anti-Human SIPA1 Polyclonal Antibody | anti-SIPA1 antibody

SIPA1, CT (SIPA1, SPA1, Signal-induced proliferation-associated protein 1, GTPase-activating protein Spa-1, p130 SPA-1)

Cat. Num. AAA646885

• Gene Names: SIPA1; SPA1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SIPA1; Polyclonal Antibody; CT (SIPA1; SPA1; Signal-induced proliferation-associated protein 1; GTPase-activating protein Spa-1; p130 SPA-1); Anti -SIPA1; anti-SIPA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SIPA1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SIPA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 941-970 amino acids from the C-terminal region of human SIPA1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SIPA1 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SIPA1 antibody detected the SIPA1 protein (arrow).)

Related Product Information for anti-SIPA1 antibody

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.

Product Categories/Family for anti-SIPA1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 206725526
• NCBI GeneID: 6494
• NCBI Accession #: NP_694985.29
• NCBI GenBank Nucleotide #: NM_153253.29
• UniProt Accession #: Q96FS4; O14518; O60484; O60618; Q2YD83
• Molecular Weight: 112,149 Da
• NCBI Official Full Name: signal-induced proliferation-associated protein 1
• NCBI Official Synonym Full Names: signal-induced proliferation-associated 1
• NCBI Official Symbol: SIPA1
• NCBI Official Synonym Symbols: SPA1
• NCBI Protein Information: signal-induced proliferation-associated protein 1; sipa-1; p130 SPA-1; GTPase-activating protein Spa-1; signal-induced proliferation-associated gene 1
• UniProt Protein Name: Signal-induced proliferation-associated protein 1
• Protein Family: Signal-induced proliferation-associated protein
• UniProt Gene Name: SIPA1
• UniProt Synonym Gene Names: SPA1; Sipa-1
• UniProt Entry Name: SIPA1_HUMAN

NCBI Description

The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state. Ref.1

Subcellular location: Nucleus. Cytoplasm › perinuclear region. Endomembrane system; Peripheral membrane protein. Note: Mostly localized in the perinuclear membraneous region.

Tissue specificity: Expressed in fetal as well as in adult tissues. Expressed abundantly in the lymphoid tissues such as thymus, spleen and peripheral blood lymphocytes and also shows a significant expression in the spinal cord.

Induction: Repressed by 12-O-tetradecanoylphorbol-13-acetate (TPA) in promyelocytic HL-60 cells.

Sequence similarities: Contains 1 PDZ (DHR) domain.Contains 1 Rap-GAP domain.