Western Blot (WB)
(WB: A431 cell lysate was probed with (1ug/ml).)
Rabbit SHP 2 Polyclonal Antibody | anti-SHP2 antibody
SHP 2 (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11) (MaxLight 490)
Gene Names
Ptpn11; Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
Reactivity
Mouse, Human, Rat
Applications
Immunocytochemistry, Western Blot, Immunoprecipitation, Neutralization Assay
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SHP 2; Polyclonal Antibody; SHP 2 (SHPTP-2; PTP2C; SH-PTP2; PTP1D; SH-PTP3; Syp; PTPN11) (MaxLight 490); anti-SHP2 antibody
Host
Rabbit
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse SHP 2/SHPTP-2 at 64kD. Apparent size increases to ~70kD upon tyrosine phosphorylation. Species Crossreactivity: human and rat.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Applicable Applications for anti-SHP2 antibody
Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP), Neutralization
Application Notes
ICC: 10ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells.
WB: 1-4ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts.
Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1ug/ml for this application.
IP: 6ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells.
Neutralization: Does not inhibit phosphotyrosine phosphatase activity.
Applications are based on unconjugated antibody.
WB: 1-4ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts.
Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1ug/ml for this application.
IP: 6ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells.
Neutralization: Does not inhibit phosphotyrosine phosphatase activity.
Applications are based on unconjugated antibody.
Immunogen
GST fusion protein corresponding to aa2-216 of mouse SHP 2/SHPTP-2.
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(WB: A431 cell lysate was probed with (1ug/ml).)
Western Blot (WB)
(WB: A431 cell lysate was probed with (1ug/ml).)
Product Categories/Family for anti-SHP2 antibody
References
1. Feng, G.S., et al., Science 259: 1607 (1993). 2. Bruhns, P., et al., J. Biol. Chem. 275: 37, 357-37, 364 (2000). 3. Rivard, N., et al., J. Biol. Chem. 270: 11, 017-11, 024 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,035 Da
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 11 isoform b
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 11
NCBI Official Symbol
Ptpn11
NCBI Official Synonym Symbols
Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 11
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 11
UniProt Gene Name
Ptpn11
UniProt Synonym Gene Names
SHP-2; Shp2
Uniprot Description
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.
Research Articles on SHP2
Similar Products
Product Notes
The SHP2 ptpn11 (Catalog #AAA6499325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHP 2 (SHPTP-2, PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp, PTPN11) (MaxLight 490) reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHP 2 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP), Neutralization. ICC: 10ug/ml detects SHP 2/SHPTP-2 in EGF stimulated A431 cells. WB: 1-4ug/ml detects SHP 2/SHPTP-2 in RIPA lysates from human A431 carcinoma cells and mouse 3T3/A31 fibroblasts. Strongly recognizes the SHP 2/SHPTP-2 fusion protein (10ng) used for immunoprecipitation; use 1ug/ml for this application. IP: 6ug immunoprecipitates SHP 2/SHPTP-2 from RIPA lysates of human A431 carcinoma cells. An immunoprecipitation phosphatase assay using phenylphosphate as a substrate was performed using EGF stimulated A431 cells. Neutralization: Does not inhibit phosphotyrosine phosphatase activity. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SHP2 ptpn11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHP 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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