SHP2 Active Protein | PTPN11 active protein

SHP2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122757

• Gene Names: Ptpn11; Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: SHP2; SHP2 Protein; Mouse; Recombinant (His Tag); Mouse SHP2/PTPN11 Protein (His Tag); 2700084A17Rik Protein; AW536184 Protein; PTP1D Protein; PTP2C Protein; SAP-2 Protein; SH-PTP2 Protein; SH-PTP3 Protein; SHP-2 Protein; Shp2 Protein; Syp Protein; protein tyrosine phosphatase; non-receptor type 11; PTPN11 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg593

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse PTPN11 (P35235-2)(Met1-Arg593) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site. The specific activity is >1pmoles/min/ug.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for PTPN11 active protein

Background: SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulation of its RhoA binding activity.

Product Categories/Family for PTPN11 active protein

ITIM/ITAM Immunoreceptors and Related Molecules Phosphatases and Regulators; B Cell Receptor Signaling Pathway

References

Ganju R K, et al. (2000) Beta-chemokine receptor CCR5 signals through SHP1, SHP2, and Syk. J Biol Chem. 275(23):17263-8.Yin T, et al. (1997) Molecular characterization of specific interactions between SHP-2 phosphatase and JAK tyrosine kinases. J Biol Chem. 272(2):1032-7.Kontaridis MI, et al. (2006) PTPN11 (Shp2) mutations in LEOPARD syndrome have dominant negative, not activating, effects. J Biol Chem. 281(10):6785-92.

NCBI and Uniprot Product Information

• NCBI GI #: 158508568
• NCBI GeneID: 19247
• NCBI Accession #: NP_001103462.1
• NCBI GenBank Nucleotide #: NM_001109992.1
• UniProt Accession #: P35235; Q3TQ84; Q64509; Q6PCL5
• Molecular Weight: 68,035 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform b
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: Ptpn11
• NCBI Official Synonym Symbols: Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: Ptpn11
• UniProt Synonym Gene Names: SHP-2; Shp2

Uniprot Description

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulatation of its RhoA binding activity. Dephosphorylates CDC73.