SHP2 / PTPN11 active protein

Recombinant Mouse SHP2 / PTPN11 Protein (His tag)

Cat. Num. AAA2546971

• Gene Names: Ptpn11; Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: SHP2 / PTPN11; Recombinant Mouse SHP2 / PTPN11 Protein (His tag); 2700084A17Rik;AW536184;PTP1D;PTP2C;SAP-2;SH-PTP2;SH-PTP3;SHP-2;Shp2;Syp; SHP2 / PTPN11 active protein

• Host: HEK293 Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyopillzed from sterle 20mM Tris, 0.1M NaCl, 10% glycerol,1Mm TCEP, 5% Trehalose, 5% Mannital, 0.01% Tween 80.
• Sequence Length: 593

• Tag: C-his
• Bio-Activity: Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site .The specific activity is > 1 pmoles/min/ug.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Reconstitution: It is recommended that sterile water (1000 ul) be added to the vial to prepare a stock solution of 0.1 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized protein should be stored at < -20°C , though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4°C to 7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for SHP2 / PTPN11 active protein

Background: SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase (PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.

NCBI and Uniprot Product Information

• NCBI GI #: 158508568
• NCBI GeneID: 19247
• NCBI Accession #: NP_001103462.1
• NCBI GenBank Nucleotide #: NM_001109992.1
• UniProt Accession #: P35235; Q3TQ84; Q64509; Q6PCL5
• Molecular Weight: AP_Mol_Mass: 65 kDa; Mol. Mass: 69.5 kDa
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 11 isoform b
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 11
• NCBI Official Symbol: Ptpn11
• NCBI Official Synonym Symbols: Syp; Shp2; PTP1D; PTP2C; SAP-2; SHP-2; SH-PTP2; SH-PTP3; AW536184; 2700084A17Rik
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 11
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 11
• UniProt Gene Name: Ptpn11
• UniProt Synonym Gene Names: SHP-2; Shp2

Uniprot Description

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 5|5 F

Cellular Component: cell junction; cytoplasm; cytosol; membrane; mitochondrion; nucleus; protein complex; stress fiber

Molecular Function: cell adhesion molecule binding; D1 dopamine receptor binding; insulin receptor binding; insulin receptor substrate binding; non-membrane spanning protein tyrosine phosphatase activity; peptide hormone receptor binding; phospholipase binding; phosphoprotein phosphatase activity; protein binding; protein domain specific binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; SH3/SH2 adaptor activity

Biological Process: abortive mitotic cell cycle; activation of MAPK activity; axonogenesis; Bergmann glial cell differentiation; brain development; cerebellar cortex formation; DNA damage checkpoint; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; genitalia development; glucose homeostasis; heart development; homeostasis of number of cells within a tissue; hormone metabolic process; hormone-mediated signaling; inner ear development; integrin-mediated signaling pathway; lipid metabolic process; microvillus organization and biogenesis; multicellular organism growth; negative regulation of cell adhesion mediated by integrin; negative regulation of cortisol secretion; negative regulation of growth hormone secretion; negative regulation of hormone secretion; negative regulation of insulin secretion; nerve growth factor receptor signaling pathway; organ growth; platelet formation; platelet-derived growth factor receptor signaling pathway; positive regulation of hormone secretion; positive regulation of mitotic cell cycle; positive regulation of signal transduction; protein amino acid dephosphorylation; regulation of cell adhesion mediated by integrin; regulation of multicellular organism growth; regulation of protein complex assembly; regulation of protein export from nucleus; reproductive process in a multicellular organism; triacylglycerol metabolic process