Rabbit anti-Human SHISA5 Polyclonal Antibody | anti-SHISA5 antibody

SHISA5, ID (SHISA5, SCOTIN, Protein shisa-5, Putative NF-kappa-B-activating protein 120, Scotin) (APC)

Cat. Num. AAA6347429

• Gene Names: SHISA5; SCOTIN
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SHISA5; Polyclonal Antibody; ID (SHISA5; SCOTIN; Protein shisa-5; Putative NF-kappa-B-activating protein 120; Scotin) (APC); Scotin; anti-SHISA5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 240

• Applicable Applications for anti-SHISA5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SHISA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-102 amino acids from the Central region of human SHISA5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SHISA5 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SHISA5 antibody detected the SHISA5 protein (arrow).)

Related Product Information for anti-SHISA5 antibody

SHISA5 can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis.

Product Categories/Family for anti-SHISA5 antibody

Antibodies; Apoptosis

References

So, H.C., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (1), 103-113 (2010): Draeby, I., et al. Arch. Biochem. Biophys. 467(1):87-94(2007). Ludes-Meyers, J.H., et al. Oncogene 23(29):5049-5055(2004). Matsuda, A., et al. Oncogene 22(21):3307-3318(2003). Bourdon, J.C., et al. J. Cell Biol. 158(2):235-246(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21703710
• NCBI GeneID: 51246
• NCBI Accession #: NP_057563.3
• NCBI GenBank Nucleotide #: NP_057563.3
• UniProt Accession #: Q8N114
• NCBI Official Full Name: protein shisa-5 isoform a
• NCBI Official Synonym Full Names: shisa family member 5
• NCBI Official Symbol: SHISA5
• NCBI Official Synonym Symbols: SCOTIN
• NCBI Protein Information: protein shisa-5
• UniProt Protein Name: Protein shisa-5
• Protein Family: Protein shisa
• UniProt Gene Name: SHISA5
• UniProt Synonym Gene Names: SCOTIN
• UniProt Entry Name: SHSA5_HUMAN

NCBI Description

This gene encodes a member of the shisa family. The encoded protein is localized to the endoplasmic reticulum, and together with p53 induces apoptosis in a caspase-dependent manner. Alternative splicing results in multiple transcript variants. Related pseudogenes of this gene are found on chromosome X. [provided by RefSeq, Apr 2016]

Uniprot Description

Function: Can induce apoptosis in a caspase-dependent manner and plays a role in p53/TP53-dependent apoptosis. Ref.1

Subunit structure: Interacts with PDCD6; PDCD6 can stabilze SHISA5. Ref.7

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus membrane Ref.1.

Induction: Induced in a p53/TP53-dependent manner in response to cellular stress. Ref.1

Domain: The proline-rich region is required for endoplasmic reticulum localization. Ref.1

Sequence similarities: Belongs to the shisa family.