NQO1 recombinant protein

NQO1, 1-274aa, Human, His tag, E Coli

Cat. Num. AAA203393

• Gene Names: NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NQO1; 1-274aa; Human; His tag; E Coli; NAD (P)H dehydrogenase quinone 1.; DHQu; DIA4; DTD; NMOR1; NMORI; QR1; Azoreductase; Quinone reductase 1; DT-diaphorase; NAD (P)H dehydrogenase; quinone 1; Azoreductase.Menadione reductase; NQO 1; QR 1; Phylloquinone reductase; Cytochrome b 5 reductase; DIA 4; Diaphorase (NADH/NADPH); Diaphorase 4 Dioxin inducible 1; DT diaphorase; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); NAD (P)H dehydrogenase quinone 1 NAD (P)H menadione oxidoreductase 1 dioxin inducible; NAD (P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD (P)H:menadione oxidoreductase 1; NAD (P)H:Quinone acceptor oxidoreductase type 1; NAD (P)H:quinone oxidoreductase 1; NAD (P)H:quinone oxireductase; NMOR 1; NMOR I.; NQO1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK
• Sequence Length: 274

• Applicable Applications for NQO1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NQO1 recombinant protein

NQO1 is a member of the NAD (P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Recombinant NQO1 protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for NQO1 recombinant protein

Redox Proteins

References

Jaiswal AK., et al. (1988) J Biol Chem. 263 (27):13572-8. ; Traver RD., et al. (1992) Cancer Res. 52 (4):797-802.;

NCBI and Uniprot Product Information

• NCBI GI #: 4505415
• NCBI GeneID: 1728
• NCBI Accession #: NP_000894
• NCBI GenBank Nucleotide #: NM_000903.2
• UniProt Accession #: P15559; Q86UK1; B2R5Y9; B4DNM7; B7ZAD1
• Molecular Weight: 33.0 kDa (294aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H quinone dehydrogenase 1
• NCBI Official Symbol: NQO1
• NCBI Official Synonym Symbols: DTD; QR1; DHQU; DIA4; NMOR1; NMORI
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: NQO1
• UniProt Synonym Gene Names: DIA4; NMOR1; DTD; QR1

NCBI Description

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.