Western Blot (WB)
(Detection of Human Chymase/CMA1 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1ug/mL of MBS601299 followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Chymase/CMA1 at ~28kD (as indicated). This experiment was conducted under reducing conditions.)
Mouse anti-Human Mast Cell Chymase Monoclonal Antibody | anti-CMA1 antibody
Mast Cell Chymase (CMA1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Direct ELISA: 0.5-1ug/ml. Detection limit for rhChymase is ~1ng/well.
Western Blot: 1-2ug/ml. Detection limit for rhChymase is
~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection wil increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml from conditioned cell culture medium.
Western Blot (WB)
(Detection of Human Chymase/CMA1 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1ug/mL of MBS601299 followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Chymase/CMA1 at ~28kD (as indicated). This experiment was conducted under reducing conditions.)
Western Blot (WB)
(Detection of Human Chymase/CMA1 by Western Blot. Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1ug/mL of MBS601299 followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Chymase/CMA1 at ~28kD (as indicated). This experiment was conducted under reducing conditions.)
NCBI and Uniprot Product Information
NCBI Description
This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq]
Uniprot Description
CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: Protease; Secreted, signal peptide; EC 3.4.21.39; Secreted
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding
Biological Process: extracellular matrix disassembly; interleukin-1 beta biosynthetic process; extracellular matrix organization and biogenesis; positive regulation of angiogenesis; cellular protein metabolic process; midbrain development; regulation of inflammatory response; protein processing; angiotensin maturation; proteolysis
Research Articles on CMA1
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Product Notes
The CMA1 cma1 (Catalog #AAA601299) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mast Cell Chymase (CMA1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mast Cell Chymase can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot and Immunoprecipitation. Dilution: Direct ELISA: 0.5-1ug/ml. Detection limit for rhChymase is ~1ng/well. Western Blot: 1-2ug/ml. Detection limit for rhChymase is ~10ng/lane under non-reducing and reducing conditions. Chemiluminescent detection wil increase sensitivity by 5 to 50 fold. Immunoprecipitation: 25ug/ml from conditioned cell culture medium. Researchers should empirically determine the suitability of the CMA1 cma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mast Cell Chymase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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