Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells using SHC2 antibody.)
Rabbit anti-Human, Rat SHC2 Polyclonal Antibody | anti-SHC2 antibody
SHC2 Antibody
Gene Names
SHC2; SCK; SLI; SHCB
Reactivity
Human, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SHC2; Polyclonal Antibody; SHC2 Antibody; SCK; SH2 domain protein C2; SHC transforming protein 2; SHCB; anti-SHC2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SHC2 antibody detects endogenous levels of total SHC2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
582
Applicable Applications for anti-SHC2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Tissue Specificity
Expressed in brain. Expressed at high level in the hypothalamus and at low level in the caudate nucleus.
Predicted Cross Reactivity
Chicken
Similarity
Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells using SHC2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from MCF-7 cells using SHC2 antibody.)
Immunofluorescene (IF)
(MBS9605960 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605960 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-SHC2 antibody
Function: Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons (By similarity).
Subunit Structure: Interacts with the Trk receptors in a phosphotyrosine-dependent manner and MEGF12. Once activated, binds to GRB2.
Post-translational Modifications: Phosphorylated on tyrosines by the Trk receptors.
Similarity: The PID domain mediates binding to the TrkA receptor.
Subunit Structure: Interacts with the Trk receptors in a phosphotyrosine-dependent manner and MEGF12. Once activated, binds to GRB2.
Post-translational Modifications: Phosphorylated on tyrosines by the Trk receptors.
Similarity: The PID domain mediates binding to the TrkA receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 59 kD
Predicted: 62 kDa
Predicted: 62 kDa
NCBI Official Full Name
SHC-transforming protein 2
NCBI Official Synonym Full Names
SHC adaptor protein 2
NCBI Official Symbol
SHC2
NCBI Official Synonym Symbols
SCK; SLI; SHCB
NCBI Protein Information
SHC-transforming protein 2
UniProt Protein Name
SHC-transforming protein 2
Protein Family
UniProt Gene Name
SHC2
UniProt Synonym Gene Names
SCK; SHCB; SH2 domain protein C2
Uniprot Description
Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons ().
Research Articles on SHC2
Similar Products
Product Notes
The SHC2 shc2 (Catalog #AAA9605960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHC2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SHC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SHC2 shc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
