Goat anti-Human PPP2R3A Polyclonal Antibody | anti-PPP2R3A antibody

PPP2R3A, ID (Serine/Threonine-protein Phosphatase 2A Regulatory Subunit B'' Subunit alpha, PP2A Subunit B Isoform PR72/PR130, PP2A Subunit B Isoform R3 Isoform, PP2A Subunit B Isoforms B''-PR72/PR130, PP2A Subunit B Isoforms B72/B130, Serine/Threonine-pro

Cat. Num. AAA647117

• Gene Names: PPP2R3A; PR72; PR130; PPP2R3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PPP2R3A; Polyclonal Antibody; ID (Serine/Threonine-protein Phosphatase 2A Regulatory Subunit B'' Subunit alpha; PP2A Subunit B Isoform PR72/PR130; PP2A Subunit B Isoform R3 Isoform; PP2A Subunit B Isoforms B''-PR72/PR130; PP2A Subunit B Isoforms B72/B130; Serine/Threonine-pro; Anti -PPP2R3A; anti-PPP2R3A antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PPP2R3A.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-PPP2R3A antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:16,000; Western Blot: 1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to C-QKDVENDGPEPSD from an internal region of human PPP2R3A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with PPP2R3A antibody at 5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PPP2R3A antibody

PP-2 C alpha/beta is heterodimer located in the mitochondrial matrix. Within the pyruvate dehydrogenase complex, PP-2 C alpha/beta serves to catalyze the dephosphorylation and concomitant reactivation of the E1 component's alpha subunit. Mutations occurring in the PDP1 gene are the cause of a disease known as pyruvate dehydrogenase deficiency (PDP deficiency), which leads to neurological dysfunction from lactic acidosis.

Product Categories/Family for anti-PPP2R3A antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 117938828
• NCBI GeneID: 5523
• UniProt Accession #: Q06190; Q06189; Q9NPQ5; A8KAE7; B4DNU1; B7ZAE3
• Molecular Weight: 130,278 Da
• NCBI Official Full Name: PPP2R3A protein
• NCBI Official Synonym Full Names: protein phosphatase 2, regulatory subunit B'', alpha
• NCBI Official Symbol: PPP2R3A
• NCBI Official Synonym Symbols: PR72; PR130; PPP2R3
• NCBI Protein Information: serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha; PP2A, subunit B, R3 isoform; PP2A subunit B isoforms B72/B130; serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B; protein phosphatase 2 (formerly 2A), regulatory subunit B'' (PR 72), alpha isoform and (PR 130), beta isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP2R3A
• UniProt Synonym Gene Names: PPP2R3
• UniProt Entry Name: P2R3A_HUMAN

NCBI Description

This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Jun 2010]

Uniprot Description

Function: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Subunit structure: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.

Tissue specificity: Expressed in heart, brain, placenta, lung, muscle and kidney.

Sequence similarities: Contains 2 EF-hand domains.