Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human HepG2 Cells.)
Rabbit anti-Human SHC-Transforming Protein 3 Polyclonal Antibody | anti-SHC3 antibody
Polyclonal Antibody to SHC-Transforming Protein 3 (SHC3)
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human HepG2 Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human HepG2 Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SHC3, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SHC3, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Kidney Tissue)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: SHC3 knockout HepG2 cell lysate; Predicted MW: 64,51kd Observed MW: 51kd Primary Ab: 3ug/ml Rabbit Anti-Human SHC3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: SHC3 knockout HepG2 cell lysate; Predicted MW: 64,51kd Observed MW: 51kd Primary Ab: 3ug/ml Rabbit Anti-Human SHC3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
Uniprot Description
SHC3: an adapter protein containing an SH2 and a PID domain. Couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons and the regulation of NMDA receptor function in the hippocampus. May modulate hippocampal synaptic plasticity underlying learning and memory. Interacts with the Trk receptors in a phosphotyrosine-dependent manner. Once activated, binds to GRB2. Interacts with activated EGF receptors. Mainly expressed in brain. Hardly detectable in other tissues, except in pancreas. Highly expressed in the cerebral cortex, frontal and temporal lobes, occipital pole, hippocampus, caudate nucleus and amygdala. Expressed at low level in the cerebellum, medulla and spinal cord. A switch from Shc1 to Shc3 occurs during maturation of neuronal precursors to postmitotic neurons. Two alternatively spliced isoforms have been described.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 9q22.1
Cellular Component: cytosol; plasma membrane
Molecular Function: phosphotyrosine binding; protein binding; Ras guanyl-nucleotide exchange factor activity; receptor tyrosine kinase binding; signal transducer activity
Biological Process: axon guidance; central nervous system development; epidermal growth factor receptor signaling pathway; learning and/or memory; MAPK cascade; Ras protein signal transduction; regulation of molecular function; synaptic transmission, glutamatergic; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on SHC3
Similar Products
Product Notes
The SHC3 shc3 (Catalog #AAA2027238) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to SHC-Transforming Protein 3 (SHC3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHC-Transforming Protein 3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the SHC3 shc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHC-Transforming Protein 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
