Rabbit DARS Polyclonal Antibody | anti-DARS antibody

DARS Polyclonal Antibody

Cat. Num. AAA3010573

• Gene Names: DARS1; DARS; HBSL; aspRS
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DARS; Polyclonal Antibody; DARS Polyclonal Antibody; Aspartate--tRNA ligase; cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein; PIG40; anti-DARS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DARS polyclonal antibody detects endogenous levels of DARS protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 401

• Applicable Applications for anti-DARS antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Recombinant full length Human DARS.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DARS polyclonal antibody)

Related Product Information for anti-DARS antibody

Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of cognate tRNA in the initial step of ribosome-dependent protein biosynthesis. DaRS is part of a multisubunit complex of aminoacyl-tRNA synthetases and is involved in the transfer of Asp-tRNA to EF-1 alpha1 (elongation factor alpha 1). The N-terminus of DaRS in vertebrates is a newly evolved structure that contains a putative amphiphilic helix and is dissimilar between different species. The N-terminal extension acts as a switch that, when in its stretched form, reduces the rate of dissociation of Asp-tRNA from DaRS, thereby providing enough time for EF-1 alpha1 to interact with Asp-tRNA. This suggests that the N-terminus of DaRS plays a critical role in its catalytic function. DaRS contains two phosphorylations sites, forms homodimers and localizes to the cytoplasm.

NCBI and Uniprot Product Information

• NCBI GI #: 648216372
• NCBI GeneID: 1615
• NCBI Accession #: NP_001280241.1
• NCBI GenBank Nucleotide #: NP_001280241.1
• UniProt Accession #: P14868
• Molecular Weight: ~ 57kDa
• NCBI Official Full Name: aspartate--tRNA ligase, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: aspartyl-tRNA synthetase 1
• NCBI Official Symbol: DARS1
• NCBI Official Synonym Symbols: DARS; HBSL; aspRS
• NCBI Protein Information: aspartate--tRNA ligase, cytoplasmic
• UniProt Protein Name: Aspartate--tRNA ligase, cytoplasmic
• UniProt Gene Name: DARS
• UniProt Synonym Gene Names: AspRS
• UniProt Entry Name: SYDC_HUMAN

NCBI Description

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

DARS: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Translation; EC 6.1.1.12; Ligase

Chromosomal Location of Human Ortholog: 2q21.3

Cellular Component: membrane; cytoplasm; cytosol

Molecular Function: protein binding; aspartate-tRNA ligase activity; ATP binding; aminoacylase activity

Biological Process: tRNA aminoacylation for protein translation; translation; aspartyl-tRNA aminoacylation; protein complex assembly; gene expression

Disease: Hypomyelination With Brainstem And Spinal Cord Involvement And Leg Spasticity