Chicken Defensin Beta 2 ELISA Kit | DEFbeta2 elisa kit

Chicken Defensin Beta 2 ELISA Kit

Cat. Num. AAA743804

• Gene Names: Defb2; BD-2
• Reactivity: Chicken
• Synonyms: Defensin Beta 2; Chicken Defensin Beta 2 ELISA Kit; DEFbeta2 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This DEFB2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken DEFB2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFbeta2 elisa kit

Principle of the assay: DEFB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DEFB2 antibody and an DEFB2-HRP conjugate. The assay sample and buffer are incubated together with DEFB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DEFB2 concentration since DEFB2 from samples and DEFB2-HRP conjugate compete for the anti-DEFB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DEFB2 from the sample, fewer sites are left to bind DEFB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DEFB2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DEFbeta2 elisa kit

Microbiology

NCBI and Uniprot Product Information

• NCBI GI #: 80478968
• NCBI GeneID: 13215
• UniProt Accession #: P82020; Q32MH3
• Molecular Weight: 7,820 Da
• NCBI Official Full Name: Defensin beta 2
• NCBI Official Synonym Full Names: defensin beta 2
• NCBI Official Symbol: Defb2
• NCBI Official Synonym Symbols: BD-2
• NCBI Protein Information: beta-defensin 2
• UniProt Protein Name: Beta-defensin 2
• UniProt Gene Name: Defb2
• UniProt Synonym Gene Names: BD-2; mBD-2
• UniProt Entry Name: DEFB2_MOUSE

Uniprot Description

Function: Has bactericidal activity

By similarity.

Subcellular location: Secreted

By similarity.

Tissue specificity: Kidney, uterus and to a lesser extent in heart. Ref.1

Induction: In tracheal epithelium, by lipopolysaccharide or inflammation. Ref.1

Sequence similarities: Belongs to the beta-defensin family.