Rabbit anti-Human SGPL1 Polyclonal Antibody | anti-SGPL1 antibody

SGPL1, NT (SGPL1, KIAA1252, Sphingosine-1-phosphate lyase 1, Sphingosine-1-phosphate aldolase) (FITC)

Cat. Num. AAA6347057

• Gene Names: SGPL1; SPL; S1PL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SGPL1; Polyclonal Antibody; NT (SGPL1; KIAA1252; Sphingosine-1-phosphate lyase 1; Sphingosine-1-phosphate aldolase) (FITC); Sphingosine-1-phosphate aldolase; anti-SGPL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SGPL1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SGPL1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping somewhere between amino acids 72-101 of human SGPL1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SGPL1 in MDA-MB435 cell line lysate (35ug/lane) using antibodyMBS6008190.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of formalin-fixed, paraffin-embedded human liver tissue using antibodyMBS6008190. Peroxidase-conjugated secondary antibody and DAB staining were used. )

Related Product Information for anti-SGPL1 antibody

SGPL1 cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis.

Product Categories/Family for anti-SGPL1 antibody

Antibodies; KIAA; orf

References

Seo, Y.J., et al. J. Virol. 84(16):8124-8131(2010). Vogel, P., et al. PLoS ONE 4 (1), E4112 (2009): Peest, U., et al. J. Cell. Biochem. 104(3):756-772(2008). Morgan, A.R., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 144B (6), 762-770 (2007): Oskouian, B., et al. Proc. Natl. Acad. Sci. U.S.A. 103(46):17384-17389(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 31982936
• NCBI GeneID: 8879
• NCBI Accession #: NP_003892.2
• NCBI GenBank Nucleotide #: NM_003901.3
• UniProt Accession #: O95470; Q7Z732; Q9ULG8; Q9UN89; B2RBD4
• Molecular Weight: 63,524 Da
• NCBI Official Full Name: sphingosine-1-phosphate lyase 1
• NCBI Official Synonym Full Names: sphingosine-1-phosphate lyase 1
• NCBI Official Symbol: SGPL1
• NCBI Official Synonym Symbols: SPL; S1PL
• NCBI Protein Information: sphingosine-1-phosphate lyase 1; hSPL; SPL 1; SP-lyase 1; sphingosine-1-phosphate aldolase
• UniProt Protein Name: Sphingosine-1-phosphate lyase 1
• Protein Family: Sphingosine-1-phosphate lyase
• UniProt Gene Name: SGPL1
• UniProt Synonym Gene Names: KIAA1252; S1PL; SP-lyase 1; SPL 1; hSPL
• UniProt Entry Name: SGPL1_HUMAN

Uniprot Description

SGPL1: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.

Protein type: Lipid Metabolism - sphingolipid; Apoptosis; EC 4.1.2.27; Membrane protein, integral; Lyase

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; sphinganine-1-phosphate aldolase activity; carboxy-lyase activity; pyridoxal phosphate binding

Biological Process: estrogen metabolic process; sphingolipid metabolic process; sphingolipid biosynthetic process; androgen metabolic process; regulation of multicellular organism growth; platelet-derived growth factor receptor signaling pathway; palate development; fatty acid metabolic process; sphingolipid catabolic process; post-embryonic development; Leydig cell differentiation; skeletal morphogenesis; luteinization; spermatogenesis; hemopoiesis; vasculogenesis; kidney development; ceramide metabolic process