Guinea pig NMNAT2 Polyclonal Antibody | anti-NMNAT2 antibody

NMNAT2 (Nicotinamide Mononucleotide Adenylyltransferase 2, NMN Adenylyltransferase 2, NaMN Adenylyltransferase 2)

Cat. Num. AAA648169

• Gene Names: NMNAT2; PNAT2; C1orf15
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NMNAT2; Polyclonal Antibody; NMNAT2 (Nicotinamide Mononucleotide Adenylyltransferase 2; NMN Adenylyltransferase 2; NaMN Adenylyltransferase 2); Anti -NMNAT2 (Nicotinamide Mononucleotide Adenylyltransferase 2; anti-NMNAT2 antibody

• Host: Guinea pig
• Clonality: Polyclonal
• Specificity: Recognizes human NMNAT2. Detects a band of ~35kD by Western blot. Not tested in other species.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.

• Applicable Applications for anti-NMNAT2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot
• Immunogen: Recombinant human NMNAT2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis using MBS648169 at 1:4'000 dilution. 1: Human NMNAT2 (His-tagged) (50ng). 2: Human mesenchymal stem cell lysate (V1T1) (50ug). 3: Unrelated Protein (His-tagged) (50ng).)

Immunohistochemistry (IHC)

(Immunohistochemical staining of NMNAT2 using MBS648169 in human brain cortex tissue (15ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Related Product Information for anti-NMNAT2 antibody

NMNAT2 catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form nicotinic acid mononucleotide (NaMN) as a substrate but with lower efficiency. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans. Has been shown to correlate with Alzheimer's disease in APPswe/PS1dE9 transgenic mice and delayed wallerian degeneration in cultured superior cervical ganglia (SCGs) from morphological changes, microtubule destruction and neurofilament degradation.

Product Categories/Family for anti-NMNAT2 antibody

Antibodies; Abs to Enzymes;  Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 25141325
• NCBI GeneID: 23057
• NCBI Accession #: NP_733820.1
• NCBI GenBank Nucleotide #: NM_170706.3
• UniProt Accession #: Q9BZQ4; O75067; Q5T1Q3; Q8WU99; Q96QW1
• Molecular Weight: 34,439 Da
• NCBI Official Full Name: nicotinamide mononucleotide adenylyltransferase 2 isoform 2
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 2
• NCBI Official Symbol: NMNAT2
• NCBI Official Synonym Symbols: PNAT2; C1orf15
• NCBI Protein Information: nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NaMN adenylyltransferase 2; pyridine nucleotide adenylyltransferase 2; nicotinate-nucleotide adenylyltransferase 2
• UniProt Protein Name: Nicotinamide mononucleotide adenylyltransferase 2
• UniProt Gene Name: NMNAT2
• UniProt Synonym Gene Names: C1orf15; KIAA0479; NMN adenylyltransferase 2
• UniProt Entry Name: NMNA2_HUMAN

NCBI Description

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity prefers NAD+, NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Ref.7 Ref.8

Catalytic activity: ATP + nicotinamide ribonucleotide = diphosphate + NAD+.ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD+.

Cofactor: Divalent metal cations. Magnesium confers the highest activity. Ref.5 Ref.6 Ref.8

Enzyme regulation: Inhibited by P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate (Np3AD) and P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate (Np4AD). Ref.8

Pathway: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.

Subunit structure: Monomer. Ref.5

Subcellular location: Cytoplasm. Golgi apparatus Ref.7.

Tissue specificity: Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans. Ref.5 Ref.6 Ref.7

Sequence similarities: Belongs to the eukaryotic NMN adenylyltransferase family.

Biophysicochemical propertiesKinetic parameters:KM is >100 µM for triazofurin monophosphate.KM=32 µM for NMN Ref.7 Ref.8KM=70 µM for NAD+KM=204 µM for ATPKM=1119 µM for PPiKM=14.5 µM for NaMNKM=304 µM for NMNHVmax=1.1 µmol/min/mg enzyme for NAD synthesisVmax=3 µmol/min/mg enzyme for pyrophosphorolytic NAD+ cleavageVmax=7 µmol/min/mg enzyme for pyrophosphorolytic NADH cleavagepH dependence:Optimum pH is 6.0-9.0.

Sequence caution: The sequence BAA32324.1 differs from that shown. Reason: Erroneous initiation.