Mouse Cytochrome P450 27B1 (CYP27B1) ELISA Kit | CYP27B1 elisa kit

Mouse Cytochrome P450 27B1 (CYP27B1) ELISA Kit

Cat. Num. AAA2020519

• Gene Names: CYP27B1; VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha
• Reactivity: Mouse
• Synonyms: Cytochrome P450 27B1 (CYP27B1); Mouse Cytochrome P450 27B1 (CYP27B1) ELISA Kit; VDD1; PDDR; CYP1; P450c1; 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase; Cytochrome P450 Family 27 Subfamily B Polypeptide 1; Calcidiol 1-monooxygenase; VD3 1A hydroxylase; CYP27B1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 27B1 (CYP27B1).; No significant cross-reactivity or interference between Cytochrome P450 27B1 (CYP27B1) and analogues was observed.
• Sequence Length: 65

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.235ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 27B1 (CYP27B1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP27B1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 27B1 (CYP27B1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 27B1 (CYP27B1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 27B1 (CYP27B1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 27B1 (CYP27B1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CYP27B1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 457866460
• NCBI GeneID: 1594
• Molecular Weight: 7,361 Da
• NCBI Official Full Name: cytochrome P450, family 27, subfamily B, polypeptide 1, partial
• NCBI Official Synonym Full Names: cytochrome P450, family 27, subfamily B, polypeptide 1
• NCBI Official Symbol: CYP27B1
• NCBI Official Synonym Symbols: VDR; CP2B; CYP1; PDDR; VDD1; VDDR; VDDRI; CYP27B; P450c1; CYP1alpha
• NCBI Protein Information: 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
• UniProt Protein Name: 25-hydroxyvitamin D3-1alpha-hydroxylase
• Protein Family: 25-hydroxyvitamin D-1 alpha hydroxylase
• UniProt Gene Name: CYP27B1
• UniProt Entry Name: Q9UP42_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP27B1: Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation. Defects in CYP27B1 are the cause of rickets vitamin D- dependent type 1A (VDDR1A); also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25- dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - steroid biosynthesis; Mitochondrial; EC 1.14.13.13; Oxidoreductase; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: mitochondrial outer membrane; mitochondrion; cytoplasm

Molecular Function: iron ion binding; heme binding; calcidiol 1-monooxygenase activity

Biological Process: steroid metabolic process; vitamin metabolic process; response to lipopolysaccharide; decidualization; bone mineralization; vitamin D metabolic process; calcium ion homeostasis; negative regulation of cell proliferation; response to vitamin D; response to estrogen stimulus; xenobiotic metabolic process; calcium ion transport; negative regulation of cell growth; regulation of bone mineralization; vitamin D catabolic process; positive regulation of keratinocyte differentiation

Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1a