SGK3 recombinant protein

SGK3 Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120956

• Gene Names: SGK3; CISK; SGK2; SGKL
• Purity: >80% as determined by SDS-PAGE
• Synonyms: SGK3; SGK3 Protein; Human; Recombinant (His & GST Tag); Human SGK3/SGKL Protein (His & GST Tag); CISK Protein; SGK2 Protein; SGKL Protein; serum/glucocorticoid regulated kinase family; member 3; SGK3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM PMSF, 10% gly, 1mM GSH, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of C
• Sequence: Met1-Leu496

• Species: Human
• Activity: No Kinase Activity
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human SGK3 (Q96BR1-1)(Met 1-Leu 496) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SGK3 recombinant protein

Background: Serine/threonine-protein kinase Sgk3, also known as Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like and SGK3, is a cytoplasmic vesicle protein that belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. SGK3 contains one AGC-kinase C-terminal domain, one protein kinase domain and one PX (phox homology) domain. Two specific sites of SGK3, one in the kinase domain (Thr-32) and the other in the C-terminal regulatory region (Ser-486), is needed to be phosphorylated for its full activation. SGK3 is expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. SGK3 is involved in the activation of potassium channels. It mediates cell IL-3-dependent survival signals. SGK3 participates in the regulation of HERG by increasing HERG protein abundance in the plasma membrane and may thus modify the duration of the cardiac action potential. SGK3 is also a very important and characteristic molecule that plays a critical role in both hair follicle morphogenesis and hair cycling.

References

Friedrich,B. et al., 2003, Pflugers Arch. 445 (6):693-6. Shojaiefard,M. et al., 2005, Biochem Biophys Res Commun. 334 (3): 742 - 6. Maier,G. et al., 2006, Cell Physiol Biochem. 18 (4-5):177-86. Okada,T. et al., 2006, Am J Pathol. 168 (4):1119-33. Mauro,T.M. et al., 2009, FASEB J 23 (9):3193-202.

NCBI and Uniprot Product Information

• NCBI GI #: 75813626
• NCBI GeneID: 23678
• NCBI Accession #: NP_001028750.1
• NCBI GenBank Nucleotide #: NM_001033578.2
• UniProt Accession #: Q96BR1; Q9P1Q7; Q9UKG5; A8K5W3; B3KQC2
• Molecular Weight: 53,306 Da
• NCBI Official Full Name: serine/threonine-protein kinase Sgk3 isoform 1
• NCBI Official Synonym Full Names: serum/glucocorticoid regulated kinase family, member 3
• NCBI Official Symbol: SGK3
• NCBI Official Synonym Symbols: CISK; SGK2; SGKL
• NCBI Protein Information: serine/threonine-protein kinase Sgk3; cytokine-independent survival kinase
• UniProt Protein Name: Serine/threonine-protein kinase Sgk3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SGK3
• UniProt Synonym Gene Names: CISK; SGKL
• UniProt Entry Name: SGK3_HUMAN

NCBI Description

This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

SGK3: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes. Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner. Induced by estrogen/ER in breast cancer cells. Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER- positive breast tumors than ER-negative breast tumors. Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser- 486), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; SGK family

Chromosomal Location of Human Ortholog: 8q12

Cellular Component: recycling endosome; cytoplasmic membrane-bound vesicle; early endosome; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; ATP binding; phosphoinositide binding; protein kinase activity

Biological Process: regulation of transcription factor activity; response to stress; regulation of cell growth; transmembrane transport; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation