Rabbit anti-Human SFTPB Polyclonal Antibody | anti-SFTPB antibody

SFTPB Antibody (Center)

Cat. Num. AAA9213975

• Gene Names: SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SFTPB; Polyclonal Antibody; SFTPB Antibody (Center); Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe); SFTP3; anti-SFTPB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SFTPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the Central region of human SFTPB.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 129-158
• Sequence Length: 393

• Applicable Applications for anti-SFTPB antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(SFTPB Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SFTPB antibody detected the SFTPB protein (arrow).)

Related Product Information for anti-SFTPB antibody

SFTPB is the pulmonary-associated surfactant
protein B (SPB), an amphipathic surfactant protein essential for
lung function and homeostasis after birth. Pulmonary surfactant is
a surface-active lipoprotein complex composed of 90% lipids and 10%
proteins which include plasma proteins and apolipoproteins SPA,
SPB, SPC and SPD. The surfactant is secreted by the alveolar cells
of the lung and maintains the stability of pulmonary tissue by
reducing the surface tension of fluids that coat the lung. The SPB
enhances the rate of spreading and increases the stability of
surfactant monolayers in vitro. Multiple mutations in this gene
have been identified, which cause pulmonary surfactant metabolism
dysfunction type 1, also called pulmonary alveolar proteinosis due
to surfactant protein B deficiency, and are associated with fatal
respiratory distress in the neonatal period. Alternatively spliced
transcript variants encoding the same protein have been identified.

Product Categories/Family for anti-SFTPB antibody

Cardiovascular; Cell Biology; Metabolism; Signal Transduction

References

Baekvad-Hansen, M., et al. Eur. Respir. J. (2010) In press :
Kim, W.J., et al. Eur. Respir. J. (2010) In press :
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Cho, M.H., et al. Respir. Res. 11, 30 (2010) :
Chaiworapongsa, T., et al. J. Matern. Fetal. Neonatal. Med. 21(9):663-670(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 288856299
• NCBI GeneID: 6439
• NCBI Accession #: NP_000533.3; NP_942140.2
• NCBI GenBank Nucleotide #: NM_000542.3
• UniProt Accession #: P07988; Q96R04
• Molecular Weight: 42117
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Synonym Full Names: surfactant protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; PSP-B; SFTB3; SFTP3; SMDP1
• NCBI Protein Information: pulmonary surfactant-associated protein B
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B
• UniProt Entry Name: PSPB_HUMAN

NCBI Description

This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]

Uniprot Description

SFTPB: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air- liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter. Defects in SFTPB are the cause of pulmonary surfactant metabolism dysfunction type 1 (SMDP1); also called pulmonary alveolar proteinosis due to surfactant protein B deficiency. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid- Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. Genetic variations in SFTPB are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS). RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. A variation Ile to Thr at position 131 influences the association between specific alleles of SFTPA1 and respiratory distress syndrome in premature infants.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p12-p11.2

Cellular Component: extracellular space; lysosome

Biological Process: organ morphogenesis; sphingolipid metabolic process; respiratory gaseous exchange

Disease: Surfactant Metabolism Dysfunction, Pulmonary, 1