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Chemiluminescence ((0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat anti-Human, Rat HTR3A Polyclonal Antibody | anti-Htr3a antibody

HTR3A (5-hydroxytryptamine Receptor 3A, 5-HT3-A, 5-HT3A, 5-hydroxytryptamine Receptor 3, 5-HT-3, 5-HT3R, Serotonin Receptor 3A, Serotonin-gated Ion Channel Receptor, 5HT3R, HTR3)

Gene Names
Htr3a; 5-HT3
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Synonyms
HTR3A; Polyclonal Antibody; HTR3A (5-hydroxytryptamine Receptor 3A; 5-HT3-A; 5-HT3A; 5-hydroxytryptamine Receptor 3; 5-HT-3; 5-HT3R; Serotonin Receptor 3A; Serotonin-gated Ion Channel Receptor; 5HT3R; HTR3); Anti -HTR3A (5-hydroxytryptamine Receptor 3A; anti-Htr3a antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Human, Rat
Clonality
Polyclonal
Specificity
Recognizes HTR3A. Species Crossreactivity: Human and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-Htr3a antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence and Western Blot.
Dilution: ELISA: 1:128,000
Western Blot: 0.3-1ug/ml, observed in human colon lysates on ~55kD bands
Immunogen
Synthetic peptide corresponding to C-GPQDFEKSPRDR, from HTR3A, at the internal region of the protein (NP_998786.2, NP_000860.2, NP_001155244.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

((0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Chemiluminescence ((0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Related Product Information for anti-Htr3a antibody
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Product Categories/Family for anti-Htr3a antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
56,056 Da
NCBI Official Full Name
Htr3a protein
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 3A
NCBI Official Symbol
Htr3a
NCBI Official Synonym Symbols
5-HT3
NCBI Protein Information
5-hydroxytryptamine receptor 3A; 5-HT-3; 5-HT3A; 5-HT3R; 5-HT3-A; serotonin receptor 3A; 5HT3 serotonin receptor; serotonin-gated ion channel receptor
UniProt Protein Name
5-hydroxytryptamine receptor 3A
UniProt Gene Name
Htr3a
UniProt Synonym Gene Names
5ht3; Htr3; 5-HT3-A; 5-HT3A; 5-HT-3; 5-HT3R
UniProt Entry Name
5HT3A_MOUSE

Uniprot Description

5-HT(3): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass; Transporter, ion channel; Channel, cation

Cellular Component: postsynaptic membrane; cell soma; membrane; axon; cytoplasm; integral to membrane; plasma membrane; synapse; cell junction

Molecular Function: serotonin binding; serotonin-activated cation-selective channel activity; voltage-gated potassium channel activity; serotonin receptor activity; ligand-gated ion channel activity; extracellular ligand-gated ion channel activity

Biological Process: transport; ion transport; serotonin receptor signaling pathway; cation transport

Research Articles on Htr3a

Similar Products

Product Notes

The Htr3a htr3a (Catalog #AAA6009187) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The HTR3A (5-hydroxytryptamine Receptor 3A, 5-HT3-A, 5-HT3A, 5-hydroxytryptamine Receptor 3, 5-HT-3, 5-HT3R, Serotonin Receptor 3A, Serotonin-gated Ion Channel Receptor, 5HT3R, HTR3) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HTR3A can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence and Western Blot. Dilution: ELISA: 1:128,000 Western Blot: 0.3-1ug/ml, observed in human colon lysates on ~55kD bands. Researchers should empirically determine the suitability of the Htr3a htr3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HTR3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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