Rabbit anti-Human, Rat HTR3A / 5-HT3A Receptor Polyclonal Antibody | anti-HTR3A antibody

Rabbit Polyclonal (IgG) to Rat HTR3A / 5-HT3A Receptor

Cat. Num. AAA241845

• Gene Names: HTR3A; HTR3; 5HT3R; 5-HT-3; 5-HT3A; 5-HT3R
• Reactivity: Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: HTR3A / 5-HT3A Receptor; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Rat HTR3A / 5-HT3A Receptor; Anti-HTR3A / 5-HT3A Receptor Antibody (aa21-36) IHC-plus; HTR3A; 5-HT3-A; 5-HT3R-A; 5-HT3 receptor; 5HT3 serotonin receptor; 5-HT3A; 5-HT3a receptor; 5-hydroxytryptamine receptor 3; 5-HT-3; 5-HT3; 5-HT3R; 5HT3R; HTR3; Serotonin receptor 3A; Rat HTR3A; 5-HT3A Receptor; anti-HTR3A antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: A mixture of synthetic peptides corresponding to amino acids 21-36 and 421-436 of rat 5-HT3 receptor
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.2% gelatin, 0.05% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 484

• Applicable Applications for anti-HTR3A antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (5 ug/ml), WB (1 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Rat
• Immunogen Description: This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949.
• Immunogen Type: Synthetic peptide
• Immunogen: HTR3A / 5-HT3A Receptor antibody was raised against this antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 21-36 (RATQAHSTTQPALLRL) and 421-436 (LSSIRHSLEKRDEMRE) of rat 5-HT3A receptor, GenBank no AAB02949. Percent identity by BLAST analysis: Rat (100%); Mouse (88%); Hamster (81%).
• Antigen Modification: aa21-36
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-5HT3A receptor antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of 5-HT3R in human brain tissue lysate with anti-5-HT3R pcAb. A protein band of approximate molecular weight of 47-50 kDa was detected.)

Related Product Information for anti-HTR3A antibody

5-Ht3a is a member of the 5-HT3 receptor subfamily of ligand gated ion channels, as opposed to all other known 5-HT receptor subtypes that are G protein-coupled receptors.

Product Categories/Family for anti-HTR3A antibody

Family: Ion channel; Subfamily: Serotonin 5-HT3 receptor

NCBI and Uniprot Product Information

• NCBI GI #: 253735700
• NCBI GeneID: 3359
• NCBI Accession #: NP_000860.2
• NCBI GenBank Nucleotide #: NM_000869.5
• UniProt Accession #: P46098; O60854; Q7KZM7; Q99918; Q9BSZ9; B4DSY6; G5E986
• Molecular Weight: 59,002 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 3A isoform b
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic
• NCBI Official Symbol: HTR3A
• NCBI Official Synonym Symbols: HTR3; 5HT3R; 5-HT-3; 5-HT3A; 5-HT3R
• NCBI Protein Information: 5-hydroxytryptamine receptor 3A; 5-HT3-A; serotonin receptor 3A; 5HT3 serotonin receptor; serotonin-gated ion channel receptor
• UniProt Protein Name: 5-hydroxytryptamine receptor 3A
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR3A
• UniProt Synonym Gene Names: 5HT3R; HTR3; 5-HT3-A; 5-HT3A; 5-HT-3; 5-HT3R
• UniProt Entry Name: 5HT3A_HUMAN

NCBI Description

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

5-HT(3): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral; Channel, ligand-gated

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: postsynaptic membrane; cell soma; axon; integral to plasma membrane; cytoplasm; plasma membrane; cell junction

Molecular Function: voltage-gated potassium channel activity; serotonin binding; serotonin-activated cation-selective channel activity; serotonin receptor activity; receptor activity

Biological Process: synaptic transmission; response to ethanol; transport; digestion; response to cocaine; transmembrane transport; serotonin receptor signaling pathway