Rabbit anti-Human Serine/Threonine-Protein Kinase ATR Polyclonal Antibody

Anti-Serine/Threonine-Protein Kinase ATR Antibody

Cat. Num. AAA4157754

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: Serine/Threonine-Protein Kinase ATR; Polyclonal Antibody; Anti-Serine/Threonine-Protein Kinase ATR Antibody; ATR protein kinase; anti-Serine/Threonine-Protein Kinase ATR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ATR protein. A BLAST analysis was used to suggest crossreactivity with ATR from human, mouse, rat, monkey, dog, fish and Xenopus sources based on a 100% homology with the immunizing sequence. Cross-reactivity with ATR from other sources has not been determined.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 2608

• Applicable Applications for anti-Serine/Threonine-Protein Kinase ATR antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 301 kDa in size corresponding to ATR by western blotting in the appropriate cell lysate or extract. Lysates from HeLa, U2OS, RAW264.7 or HEK293 cells are suggested for western blotting. ATR is ubiquitously expressed, with highest expression levels in the testes. Note that isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-Serine/Threonine-Protein Kinase ATR antibody

Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNAPK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during Sphase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

NCBI and Uniprot Product Information

• NCBI GI #: 1685610177
• NCBI GenBank Nucleotide #: XP_029349910.1
• NCBI Official Full Name: serine/threonine-protein kinase ATR
• Protein Family: Serine/threonine-protein kinase