Rabbit anti-Human ATR Polyclonal Antibody | anti-ATR antibody

Phospho-ATR (Ser428) Antibody

Cat. Num. AAA9612974

• Gene Names: ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: ATR; Polyclonal Antibody; Phospho-ATR (Ser428) Antibody; Ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; ATR_HUMAN; FCTCS; FRAP Related Protein 1; FRAP-related protein 1; FRP1; MEC1; MEC1 mitosis entry checkpoint 1 homolog; Protein kinase ATR; RAC3; Rad3 related protein; SCKL; SCKL1; Serine/threonine protein kinase ATR; Serine/threonine-protein kinase ATR; anti-ATR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-ATR (Ser428) Antibody detects endogenous levels of ATR only when phosphorylated at Ser428.; Tissue Specificity: Ubiquitous, with highest expression in testis. Isoform 2 is found in pancreas, placenta and liver but not in heart, testis and ovary.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ATR antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ATR around the phosphorylation site of Ser428.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated; autophosphorylates in vitro.
• Subunit Structure: Interacts with ATRIP; forming a heterodimer with ATRIP. Binds to DNA, and to UV-damaged DNA with higher affinity. Interacts with RAD17, MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN and CEP164. Interacts with TELO2 and TTI1.
• Similarity: Belongs to the PI3/PI4-kinase family. ATM subfamily.
• Subcellular Location: Nucleus. Nucleus>PML body. Chromosome.; Note: Depending on the cell type, it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells(heat shock treatment), using Phospho-ATR (Ser428) Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-ATR antibody

Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

NCBI and Uniprot Product Information

• NCBI GI #: 157266317
• NCBI GeneID: 545
• NCBI Accession #: NP_001175.2
• NCBI GenBank Nucleotide #: NM_001184.3
• UniProt Accession #: Q13535; Q59HB2; Q7KYL3; Q93051; Q9BXK4
• Molecular Weight: 301,367 Da
• NCBI Official Full Name: serine/threonine-protein kinase ATR
• NCBI Official Synonym Full Names: ataxia telangiectasia and Rad3 related
• NCBI Official Symbol: ATR
• NCBI Official Synonym Symbols: FRP1; MEC1; SCKL; FCTCS; SCKL1
• NCBI Protein Information: serine/threonine-protein kinase ATR; Rad3 related protein; FRAP-related protein 1; FRAP-related protein-1; MEC1, mitosis entry checkpoint 1, homolog; ataxia telangiectasia and Rad3-related protein
• UniProt Protein Name: Serine/threonine-protein kinase ATR
• Protein Family: Atrophin
• UniProt Gene Name: ATR
• UniProt Synonym Gene Names: FRP1
• UniProt Entry Name: ATR_HUMAN

NCBI Description

The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq, Jul 2008]

Uniprot Description

ATR: a ser/thr kinase of the PIKK family most closely related to ATM. Activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]Q. Phosphorylates BRCA1, Chk1, MCM2, RAD17, RPA2, SMC1 and p53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Protein type: Kinase, lipid; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Membrane protein, integral; Protein kinase, atypical; Kinase, protein; ATYPICAL group; PIKK family; ATR subfamily

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: XY body; nucleoplasm; Golgi apparatus; PML body; chromosome

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; MutLalpha complex binding; MutSalpha complex binding; ATP binding; protein kinase activity

Biological Process: response to drug; peptidyl-serine phosphorylation; negative regulation of DNA replication; multicellular organismal development; protein amino acid autophosphorylation; regulation of protein binding; DNA damage checkpoint; positive regulation of DNA damage response, signal transduction by p53 class mediator; DNA replication; cell cycle; DNA repair; response to DNA damage stimulus; double-strand break repair via homologous recombination

Disease: Seckel Syndrome 1; Cutaneous Telangiectasia And Cancer Syndrome, Familial