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Immunohistochemistry (IHC) (Anti-SEPT3 / Septin 3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Goat SEPT3 / Septin 3 Polyclonal Antibody | anti-SEPT3 antibody

Goat Polyclonal to Human SEPT3 / Septin 3

Gene Names
SEPT3; SEP3; bK250D10.3
Reactivity
Bat, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Gibbon, Xenopus (at least 90% immunogen sequence identity)
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
SEPT3 / Septin 3; Polyclonal Antibody; Goat Polyclonal to Human SEPT3 / Septin 3; Anti-SEPT3 / Septin 3 Antibody (N-Terminus) IHC-plus; SEPT3; BK250D10.3; Neuronal-specific septin 3; SEP3; Neuronal-specific septin-3; Septin 3; Human SEPT3; anti-SEPT3 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bat, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Predicted Reactivity: Gibbon, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Specificity
Human SEPT3 / Septin 3. This antibody is expected to recognize both reported isoforms according to NP_663786.1 and NP_061979.3.
Purity/Purification
Immunoaffinity Purified
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
350
Applicable Applications for anti-SEPT3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (3.75 ug/ml), WB (0.5 - 2 ug/ml)
Target Species
Human
Immunogen Description
Synthetic peptide SELVPEPRPKPA-C from the N-terminus of human SEPT3 / Septin 3 (NP_663786.1; NP_061979.3). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%).
Immunogen Type
Synthetic peptide
Immunogen
SEPT3 / Septin 3 antibody was raised against synthetic peptide SELVPEPRPKPA-C from the N-terminus of human SEPT3 / Septin 3 (NP_663786.1; NP_061979.3). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Gibbon, Platypus, Xenopus (92%); Lizard (83%).
Antigen Modification
N-Terminus
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SEPT3 / Septin 3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC) (Anti-SEPT3 / Septin 3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SEPT3 / Septin 3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC) (Anti-SEPT3 / Septin 3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SEPT3 / Septin 3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC) (Anti-SEPT3 / Septin 3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Western Blot (WB)

(Antibody staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

Western Blot (WB) (Antibody staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,061 Da
NCBI Official Full Name
neuronal-specific septin-3 isoform B
NCBI Official Synonym Full Names
septin 3
NCBI Official Symbol
SEPT3
NCBI Official Synonym Symbols
SEP3; bK250D10.3
NCBI Protein Information
neuronal-specific septin-3; neuronal-specific septin 3
UniProt Protein Name
Neuronal-specific septin-3
UniProt Gene Name
SEPT3
UniProt Synonym Gene Names
SEP3
UniProt Entry Name
SEPT3_HUMAN

NCBI Description

This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

SEPT3: belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. This gene encodes three transcript variants resulting in three distinct isoforms.

Protein type: Hydrolase; Cell cycle regulation; Cytoskeletal

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytoskeleton; cytoplasm; synapse; cell junction

Molecular Function: GTP binding

Biological Process: cell division; cell cycle

Research Articles on SEPT3

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Product Notes

The SEPT3 sept3 (Catalog #AAA243091) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human SEPT3 / Septin 3 reacts with Bat, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat Predicted Reactivity: Gibbon, Xenopus (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. AAA Biotech's SEPT3 / Septin 3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (3.75 ug/ml), WB (0.5 - 2 ug/ml). Researchers should empirically determine the suitability of the SEPT3 sept3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SEPT3 / Septin 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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