SORCS1 recombinant protein

SorCS1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121025

• Gene Names: SORCS1; hSorCS
• Purity: >92% as determined by SDS-PAGE
• Synonyms: SorCS1; SorCS1 Protein; Human; Recombinant (His Tag); Human SorCS1 Protein (His Tag); hSorCS Protein; RP11-446H13.1 Protein; sortilin-related VPS10 domain containing receptor 1; SORCS1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ser111-Ser1099

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 111
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SORCS1 (Q8WY21-1) extracellular domain (Ser 111-Ser 1099) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SORCS1 recombinant protein

Background: VPS1 domain-containing receptor SorCS1, also known as SORCS1 and SORCS, is a single-pass type I membrane protein that belongs to the SORCS family and SORCS1 subfamily. SORCS1 contains five BNR repeats and one PKD domain. SorCS1 is a member of the Vps1p-domain receptor family comprised of Sortilin, SorCS1, SorCS2, SorCS3, and SorLA. The common characteristic of these receptors is an N-terminal Vps1p domain, which either represents the only module of the luminal/extracellular moiety or is combined with additional domains. Family members play roles in protein transport and signal transduction. The individual receptors bind and internalize a variety of ligands, such as neuropeptides and trophic factors, and Sortilin and SorLA mediate trans-Golgi network-to-endosome sorting. Their prominent neuronal expression, several of the identified ligands, and results support the notion that members of this receptor family have important functions in neurogenesis, plasticity-related processes, and functional maintenance of the nervous system. Sortilin and SorLA mediate intracellular protein trafficking and sorting. SorCS1 binds platelet-derived growth factor-BB (PDGF-BB) and is expressed in isoforms differing only in their cytoplasmic domains. SorCS1 binds platelet-derived growth factor, a growth factor crucial for pericyte recruitment to the microvasculature, and may thus have a role in expanding or maintaining the islet vasculature.

References

Hermey,G. et al., 2001, Neurosci Lett. 313 (1-2): 83-7. Clee,S.M. et al., 2006, Nat Genet. 38 (6): 688-93. Goodarzi,M.O. et al., 2007, Diabetes. 56 (7):1922-9. Hermey,G. 2009, Cell Mol Life Sci. 66 (16):2677-89. Reitz,C. et al., 2011, Ann Neurol. 69 (1):47-64.

NCBI and Uniprot Product Information

• NCBI GI #: 61743975
• NCBI GeneID: 114815
• NCBI Accession #: NP_001013049.1
• NCBI GenBank Nucleotide #: NM_001013031.2
• UniProt Accession #: Q8WY21; Q59GG7; Q5JVT7; Q5JVT8; Q5VY14; Q86WQ1; Q86WQ2; Q9H1Y1; Q9H1Y2; A2RRF4
• Molecular Weight: 129,635 Da
• NCBI Official Full Name: VPS10 domain-containing receptor SorCS1 isoform b
• NCBI Official Synonym Full Names: sortilin-related VPS10 domain containing receptor 1
• NCBI Official Symbol: SORCS1
• NCBI Official Synonym Symbols: hSorCS
• NCBI Protein Information: VPS10 domain-containing receptor SorCS1; VPS10 domain receptor protein SORCS 1
• UniProt Protein Name: VPS10 domain-containing receptor SorCS1
• Protein Family: VPS10 domain-containing receptor
• UniProt Gene Name: SORCS1
• UniProt Synonym Gene Names: SORCS; hSorCS
• UniProt Entry Name: SORC1_HUMAN

NCBI Description

This gene encodes one family member of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins. The VPS10 domain name comes from the yeast carboxypeptidase Y sorting receptor Vps10 protein. Members of this gene family are large with many exons but the CDS lengths are usually less than 3700 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. These genes are strongly expressed in the central nervous system. Two of the five family members (sortilin and sortilin-related receptor) are synthesized as preproproteins; it is not yet known if this encoded protein is also a preproprotein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SORCS1: one family member of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins. The VPS10 domain name comes from the yeast carboxypeptidase Y sorting receptor Vps10 protein. Members of this gene family are large with many exons but the CDS lengths are usually less than 3700 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. These genes are strongly expressed in the central nervous system. Two of the five family members (sortilin and sortilin-related receptor) are synthesized as preproproteins; it is not yet known if this encoded protein is also a preproprotein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 10q23-q25

Cellular Component: membrane; integral to membrane

Molecular Function: protein binding; neuropeptide receptor activity

Biological Process: neuropeptide signaling pathway