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Western Blot
(Anti-C6orf58 Antibody (Center) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human C6orf58 Polyclonal Antibody | anti-C6orf58 antibody
C6orf58 Antibody (Center)
Gene Names
C6orf58; LEG1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
C6orf58; Polyclonal Antibody; C6orf58 Antibody (Center); UPF0762 protein C6orf58; anti-C6orf58 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This C6orf58 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-233 amino acids from the Central region of human C6orf58.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
205-233
Sequence Length
330
Applicable Applications for anti-C6orf58 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
May be involved in early liver development.
Cellular Location
Secreted
Tissue Location
Detected in saliva and in hypomineralized dental enamel (at protein level).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot
(Anti-C6orf58 Antibody (Center) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot
(Anti-C6orf58 Antibody (Center) at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(C6orf58 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the C6orf58 antibody detected the C6orf58 protein (arrow).)
Western Blot (WB)
(C6orf58 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the C6orf58 antibody detected the C6orf58 protein (arrow).)
Immunohistochemistry (IHC)
(C6orf58 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the C6orf58 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(C6orf58 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of the C6orf58 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(C6orf58 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(C6orf58 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-C6orf58 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37926
NCBI Official Full Name
protein LEG1 homolog
NCBI Official Synonym Full Names
chromosome 6 open reading frame 58
NCBI Official Symbol
C6orf58
NCBI Official Synonym Symbols
LEG1
NCBI Protein Information
protein LEG1 homolog
UniProt Protein Name
Protein LEG1 homolog
UniProt Gene Name
LEG1
UniProt Entry Name
LEG1H_HUMAN
Uniprot Description
C6orf58: Belongs to the UPF0762 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6q22.33
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Product Notes
The C6orf58 leg1 (Catalog #AAA9208693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 205-233. The C6orf58 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C6orf58 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the C6orf58 leg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C6orf58, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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